Summary of Study ST002529

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002529
Study TitleIn vivo 15N2-glutamine tracing by jugular vein infusion in PDAC-tumor bearing Lyz2-Arg1 and control mice
Study SummaryWe assessed the relative levels of urea cycle related metabolites in PDAC tumors in Lyz2-Cre+/+; Arg1fl/fl compared to Arg1fl/fl control host animals.
Institute
University of Chicago
Last NameApiz Saab
First NameJuan
Address929 E. 57th St.
Emailjapizsaab@uchicago.edu
Phone7738346506
Submit Date2023-03-24
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-04-13
Release Version1
Juan Apiz Saab Juan Apiz Saab
https://dx.doi.org/10.21228/M8D434
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004163
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Waters Atlantis BEH Z-HILIC (150 x 2.1 mm, 2.5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap IQ-X Tribrid
Ion Mode UNSPECIFIED
Units Natural abundance corrected percentages

Chromatography:

Chromatography ID:CH003082
Instrument Name:Thermo Vanquish
Column Name:Waters Atlantis BEH Z-HILIC (150 x 2.1 mm, 2.5um)
Column Temperature:30°C
Flow Gradient:0 minute: 85% B, 0.5 minute: 85% B, 18 minutes: 20% B, 20 minutes: 20% B, 20.5 minutes: 85% B and 28 minutes: 85% B
Flow Rate:0.2 mL/minute
Solvent A:20 mM ammonium bicarbonate at pH 9.6, adjusted by ammonium hydroxide addition
Solvent B:100% Acetonitrile
Chromatography Type:HILIC
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