Summary of Study ST002529

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002529
Study Title	In vivo 15N2-glutamine tracing by jugular vein infusion in PDAC-tumor bearing Lyz2-Arg1 and control mice
Study Summary	We assessed the relative levels of urea cycle related metabolites in PDAC tumors in Lyz2-Cre+/+; Arg1fl/fl compared to Arg1fl/fl control host animals.
Institute	University of Chicago
Last Name	Apiz Saab
First Name	Juan
Address	929 E. 57th St.
Email	japizsaab@uchicago.edu
Phone	7738346506
Submit Date	2023-03-24
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-04-13
Release Version	1

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Treatment:

Treatment ID:	TR002641
Treatment Summary:	6~8-month-old female & male Lyz2-Cre +/+; Arg1fl/fl and litter mate control Arg1fl/fl mice with murine PDAC orthotopic tumors underwent dual jugular vein & carotid artery catheterization surgery. On day 5 of post recovery, mice received a 0.28 mg/g 10 min. bolus followed by a continuous 4 hr. infusion 0.005 mg/g/min infusion of 15N2-glutamine (Cambridge Isotope Laboratory #NLM-1328-PK). Plasma samples were taken at time points: 0' 15' 30' 60' 120' 180’ 240’. Tumors and tissues were harvested at 240’ and immediately snap frozen with liquid nitrogen stored at -80°C prior to analysis.

Treatment ID:

TR002641

Treatment Summary:

6~8-month-old female & male Lyz2-Cre +/+; Arg1fl/fl and litter mate control Arg1fl/fl mice with murine PDAC orthotopic tumors underwent dual jugular vein & carotid artery catheterization surgery. On day 5 of post recovery, mice received a 0.28 mg/g 10 min. bolus followed by a continuous 4 hr. infusion 0.005 mg/g/min infusion of 15N2-glutamine (Cambridge Isotope Laboratory #NLM-1328-PK). Plasma samples were taken at time points: 0' 15' 30' 60' 120' 180’ 240’. Tumors and tissues were harvested at 240’ and immediately snap frozen with liquid nitrogen stored at -80°C prior to analysis.