Summary of Study ST002569

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001656. The data can be accessed directly via it's Project DOI: 10.21228/M8ND9B This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002569
Study TitleLipidomic analysis in very-long chain acyl-CoA dehydrogenase null mice
Study SummaryCompared to their littermate counterparts, HFD/fasted VLCAD-/- mouse hearts displayed higher VLC-acylcarnitines accumulation, higher levels of arachidonic acid (AA) and lower docosahexaenoic acid (DHA) contents in glycerophospholipids (GPLs).
Institute
Montreal Heart Institute
Last NameForest
First NameAnik
Address5000 Bélanger Street
Emailmetabolomique.icm@mhi-rc.org
Phone5143763330 ext 2133
Submit Date2023-04-11
Analysis Type DetailLC-MS
Release Date2023-09-06
Release Version1
Anik Forest Anik Forest
https://dx.doi.org/10.21228/M8ND9B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004233
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units Counts

Chromatography:

Chromatography ID:CH003141
Methods Filename:LC-MS and data processing protocol of untargeted lipidomic analysis
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0–2 min, 50% B; 2–11 min, 50–85% B; 11–33 min, 85–94% B; 33–35 min, 94–98% B, 35–38 min; 98–99.5% B; 38–82 min, 98–99.5% B; 82–83 min, 99.5–50% B, plus an equilibration of 8 min
Flow Rate:0.45 ml/min
Solvent A:100% water; 0.2% formic acid; 10 mM ammonium formate
Solvent B:55% ethanol,/35% acetonitrile/10% MTBE, 55:35:10; 0.2% formic acid; 10 mM ammonium formate
Chromatography Type:Reversed phase
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