Summary of Study ST002569

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001656. The data can be accessed directly via it's Project DOI: 10.21228/M8ND9B This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002569
Study TitleLipidomic analysis in very-long chain acyl-CoA dehydrogenase null mice
Study SummaryCompared to their littermate counterparts, HFD/fasted VLCAD-/- mouse hearts displayed higher VLC-acylcarnitines accumulation, higher levels of arachidonic acid (AA) and lower docosahexaenoic acid (DHA) contents in glycerophospholipids (GPLs).
Institute
Montreal Heart Institute
Last NameForest
First NameAnik
Address5000 Bélanger Street
Emailmetabolomique.icm@mhi-rc.org
Phone5143763330 ext 2133
Submit Date2023-04-11
Analysis Type DetailLC-MS
Release Date2023-09-06
Release Version1
Anik Forest Anik Forest
https://dx.doi.org/10.21228/M8ND9B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002676
Sampleprep Summary:Total lipids were extracted from 40mg of pulverized hearts under liquid nitrogen using in methyl tert-butyl ether (MTBE) and ethyl acetate after spiking with the following internal standards lysophosphatidylcholine (LPC) 13:0, phosphatidylcholine (PC) 19:0/19:0, PC14:0/14:0, phosphatidylserine (PS) 12:0/12:0, phosphatidylglycerol (PG) 15:0/15:0 and phosphatidylethanolamine (PE) 17:0/17:0). Supernatants were evaporated using a Speed Vacuum concentrator overnight, dissolved in methanol/chloroform (2:1) and aliquots (50 µL) were stored at -80ºC until use.
Sampleprep Protocol Filename:Sample preparation protocol of untargeted lipidomic analysis
Processing Storage Conditions:-80℃
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