Summary of Study ST002729
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001693. The data can be accessed directly via it's Project DOI: 10.21228/M8VT66 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002729 |
| Study Title | Improved Endurance Capacity of Diabetic Mice during SGLT2 Inhibition: Potential Role of AICARP, an Endogenous AMPK Activator. |
| Study Summary | Diabetes is associated with an increased risk of deleterious changes in muscle mass and function or sarcopenia, leading to physical inactivity and worsening glycemic control. Given the negative energy balance during sodium-glucose cotransporter 2 (SGLT2) inhibition, whether SGLT2 inhibitors affect skeletal muscle mass and function is a matter of concern. However, how SGLT2 inhibition affects the skeletal muscle function in patients with diabetes remains insufficiently explored. We aimed to explore the effects of canagliflozin (CANA), an SGLT2 inhibitor, on skeletal muscles in genetically diabetic db/db mice. |
| Institute | Medical Institute of Bioregulation, Kyushu University |
| Last Name | Takahashi |
| First Name | Masatomo |
| Address | Maidashi 3-1-1, Higashi-ku, Fukuoka, Fukuoka, 8128582, Japan |
| m-takahashi@bioreg.kyushu-u.ac.jp | |
| Phone | 0926426171 |
| Submit Date | 2023-05-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, mzXML, lcd |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-09 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004423 | AN004424 | AN004425 | AN004426 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Ion exchange | Reversed phase | Reversed phase | Normal phase |
| Chromatography system | Thermo Dionex ICS-5000+ | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera UC |
| Column | Dionex IonPac AS11-HC (250 x 2mm,4um) | Merck Discovery HS F5 (PFP) (150 x 2.1mm,3um) | GL Sciences Inert Sustain C18 (150 × 2.1mm, 3um) | Waters ACQUITY UPC2 Torus DEA (100 x 3.0mm, 1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Triple quadrupole |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Shimadzu LCMS-8060 |
| Ion Mode | UNSPECIFIED | UNSPECIFIED | POSITIVE | UNSPECIFIED |
| Units | relative peak area | relative peak area | concentration (pmol/mg-protein) | relative peak area |
Chromatography:
| Chromatography ID: | CH003322 |
| Chromatography Summary: | Anionic polar metabolites (i.e., organic acids, sugar phosphates, nucleotides, etc.) were analyzed via IC/HRMS/MS. |
| Instrument Name: | Thermo Dionex ICS-5000+ |
| Column Name: | Dionex IonPac AS11-HC (250 x 2mm,4um) |
| Column Temperature: | 30℃ |
| Flow Gradient: | 10-100%A, 0-24.0 min, 100%A, 24.0-27.0 min, 100-10%A, 27.0-27.1 min, 10%A, 27.1-35.0 min |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100 mM KOH in H2O |
| Solvent B: | 1 mM ammonium acetate in MeOH |
| Chromatography Type: | Ion exchange |
| Chromatography ID: | CH003323 |
| Chromatography Summary: | Cationic polar metabolites (i.e., amino acids, bases, nucleosides, etc) were analyzed via PFPP-LC/HRMS/MS. |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Merck Discovery HS F5 (PFP) (150 x 2.1mm,3um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0%B, 0-5.0 min, 0-40%B, 5.0-15.0 min, 40-100%B, 15.0-15.1 min, 100%B, 15.1-18.0 min, 100-0%B, 18.0-18.1 min, 0%B, 18.1-25.0 min |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 0.1% formic acid in H2O |
| Solvent B: | ACN |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003324 |
| Chromatography Summary: | Acyl-CoA and acyl-carnitine were analyzed via C18-LC/HRMS/MS. |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | GL Sciences Inert Sustain C18 (150 × 2.1mm, 3um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0-13.0 min, 2-95%B, 13.0-20.0 min, 95%B, 20.0-20.1 min, 95-2%B, 20.1-25.0 min, 2%B |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 5 mM ammonium acetate in H2O |
| Solvent B: | ACN |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003325 |
| Chromatography Summary: | Fatty acids (FA), diacylglycerols (DG), and triacylglycerols (TG) were analyzed via Normal phase SFC/MS/MS. |
| Instrument Name: | Shimadzu Nexera UC |
| Column Name: | Waters ACQUITY UPC2 Torus DEA (100 x 3.0mm, 1.7um) |
| Column Temperature: | 50℃ |
| Flow Gradient: | 0-1.0 min, 1%B, 1.0-24.0 min, 1-75%B, 24.0-26.0 min, 75%B, 26.0-26.1 min, 75-1%B, 26.1-30.0 min, 1%B |
| Flow Rate: | 1.0 mL/min |
| Solvent A: | CO2 |
| Solvent B: | 0.1% (w/v) ammonium acetate in MeOH/H2O (95/5) |
| Chromatography Type: | Normal phase |
| Solvent C: | 0.1% (w/v) ammonium acetate in MeOH/H2O (95/5) |
