Summary of Study ST002785
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001736. The data can be accessed directly via it's Project DOI: 10.21228/M89Q7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002785 |
| Study Title | Metabolic changes of the human kidney during isolated normothermic perfusion with red blood cell based perfusate - perfusate |
| Study Type | Experimental |
| Study Summary | This study investigates how glucose, lactate and 20 amino acids in the perfusate of normothermically perfused human kidneys that were not suitable for transplantation changes over time. |
| Institute | KU Leuven |
| Department | Microbiology, Immunology and Transplantation |
| Laboratory | Lab of Abdominal Transplantation |
| Last Name | Jochmans |
| First Name | Ina |
| Address | Herestraat 49, Leuven, 3000, Belgium |
| ina.jochmans@kuleuven.be | |
| Phone | None |
| Submit Date | 2023-07-17 |
| Num Groups | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-08-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004532 |
|---|---|
| Chromatography ID | CH003405 |
| MS ID | MS004279 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity |
| Column | Agilent InfinityLab Poroshell 120 (2.1 x 150 mm, 2.7 um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003405 |
| Chromatography Summary: | 10 µl was loaded onto an Ultra Performance Liquid Chromatograph (UPLC) equipped with a Hydrophilic Interaction Liquid Chromatography (HILIC) column (InfinityLab Poroshell 120 HILIC-Z PEEK-lined 2.1 x 150 mm, 2.7 µm column; Agilent, Santa Clara, USA)) and connected in-line to a Q-exactive Orbitrap Focus (Thermo Fisher Scientific, Massachusetts, USA) mass spectrometer. A linear gradient was built up starting with 90% solvent A (LC-MS grade acetonitrile, Acetonitrile hypergrade for LC-MS LiChrosolv, Supelco (Merck), Germany) and 10% solvent B (10 mM ammonium acetate (LiChropur™, eluent additive for LC-MS, (Merck), Germany), pH 9.3). At 2 min the gradient increased to 60% of solvent B and maintained at 60% until 15 min. The gradient returned to 10% solvent B at 16 min and remained until 25 min. The flow rate was 250 µl/min and the column was kept at 25°C throughout the analysis. The MS operated in negative ion mode, with a spray voltage of 2.9 kV and a temperature of the capillary of 325 °C. Gas settings were as follows: sheath gas 40 and auxiliary gas 15. The vaporizer temperature was set at 300 °C. A full scan (resolution of 70.000 and scan range of m/z 70-1050) was applied. XCalibur version to operate the LC-MS was 4.2.47. |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent InfinityLab Poroshell 120 (2.1 x 150 mm, 2.7 um) |
| Column Temperature: | 25°C |
| Flow Gradient: | A linear gradient was built up starting with 90% solvent A and 10% solvent B. At 2 min the gradient increased to 60% of solvent B and maintained at 60% until 15 min. |
| Flow Rate: | 250 µl/min |
| Solvent A: | 100% acetonitrile |
| Solvent B: | 100% water; 10 mM ammonium acetate, pH 9.3 |
| Chromatography Type: | HILIC |
