Summary of Study ST002785
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001736. The data can be accessed directly via it's Project DOI: 10.21228/M89Q7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002785 |
| Study Title | Metabolic changes of the human kidney during isolated normothermic perfusion with red blood cell based perfusate - perfusate |
| Study Type | Experimental |
| Study Summary | This study investigates how glucose, lactate and 20 amino acids in the perfusate of normothermically perfused human kidneys that were not suitable for transplantation changes over time. |
| Institute | KU Leuven |
| Department | Microbiology, Immunology and Transplantation |
| Laboratory | Lab of Abdominal Transplantation |
| Last Name | Jochmans |
| First Name | Ina |
| Address | Herestraat 49, Leuven, 3000, Belgium |
| ina.jochmans@kuleuven.be | |
| Phone | None |
| Submit Date | 2023-07-17 |
| Num Groups | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-08-07 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002898 |
| Sampleprep Summary: | Samples were extracted in an 80% methanol (80:20 methanol:water) (Methanol ≥99.9%, HiPerSolv CHROMANORM®, ULTRA for LC-MS, suitable for UPLC/UHPLC-MS instruments, VWR, Belgium) extraction buffer containing 1 µM of deuterated D27 myristic acid, 5 µM D12 glucose, 3 µM 13C5-D5-15N Glutamic acid and 3 µM D7-15N4-Arginine as internal standards. 10 µl of sample was added to 990 µl of the extraction buffer and stored overnight at -80 °C. Insolubilities and precipitated proteins were removed by centrifugation at 20.000 g, for 15 min at 4 °C. 200 µL of the supernatant was transferred to an appropriate mass-spectrometry vial. 10 µl of sample was added to 990 µl of the extraction buffer and stored overnight at -80 °C. |
| Extract Storage: | -80℃ |
