Summary of Study ST002925
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001817. The data can be accessed directly via it's Project DOI: 10.21228/M8V72T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002925 |
| Study Title | Metabolite analysis of hepatic Pptc7-ko mice under fed or fasting conditions |
| Study Summary | Hepatic Pptc7 knockout leads to mitochondrial loss under fed conditions in adult mice, which is exacerbated upon fasting. Under fasting conditions, WT liver upregulates Pptc7 levels to repress mitophagy and maintain mitochondrial mass. However, Pptc7-KO liver accumulated higher levels of Bnip3, resulting in mitophagy activation and further mitochondrial loss. To explore the metabolic changes upon Pptc7 knockout under both fed and fasting conditions, we performed a metabolite analysis of wild-type or Pptc7-KO mouse liver samples in this study. |
| Institute | National Institute of Biological Sciences, Beijing |
| Last Name | Ma |
| First Name | Yan |
| Address | Zhongguancun Life Science Park |
| mayan@nibs.ac.cn | |
| Phone | +86-01080726688 |
| Submit Date | 2023-10-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004796 | AN004797 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish UHPLC | Thermo Vanquish UHPLC |
| Column | Merck ZIC-HILIC (2.1x100 mm, 3.5 μm) | Merck ZIC-HILIC (2.1x100 mm, 3.5 μm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | count | count |
Chromatography:
| Chromatography ID: | CH003627 |
| Instrument Name: | Thermo Vanquish UHPLC |
| Column Name: | Merck ZIC-HILIC (2.1x100 mm, 3.5 μm) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-5 min, 99% B; 5-20 min, 99-20% B; 20-21 min, 20-99% B; 21-25 min, 99% B. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 5% acetonitrile/95% water; 10 mM ammonium acetate |
| Solvent B: | 95% acetonitrile/5% water; 10 mM ammonium acetate |
| Chromatography Type: | HILIC |
