Summary of Study ST002925
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001817. The data can be accessed directly via it's Project DOI: 10.21228/M8V72T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002925 |
| Study Title | Metabolite analysis of hepatic Pptc7-ko mice under fed or fasting conditions |
| Study Summary | Hepatic Pptc7 knockout leads to mitochondrial loss under fed conditions in adult mice, which is exacerbated upon fasting. Under fasting conditions, WT liver upregulates Pptc7 levels to repress mitophagy and maintain mitochondrial mass. However, Pptc7-KO liver accumulated higher levels of Bnip3, resulting in mitophagy activation and further mitochondrial loss. To explore the metabolic changes upon Pptc7 knockout under both fed and fasting conditions, we performed a metabolite analysis of wild-type or Pptc7-KO mouse liver samples in this study. |
| Institute | National Institute of Biological Sciences, Beijing |
| Last Name | Ma |
| First Name | Yan |
| Address | Zhongguancun Life Science Park |
| mayan@nibs.ac.cn | |
| Phone | +86-01080726688 |
| Submit Date | 2023-10-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-26 |
| Release Version | 1 |
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Project:
| Project ID: | PR001817 |
| Project DOI: | doi: 10.21228/M8V72T |
| Project Title: | A mitophagy sensor PPTC7 integrates homeostatic and physiological signals to regulate mitophagy and mitochondrial mass |
| Project Summary: | Mitophagy mediated by receptors BNIP3 and NIX critically regulates mitochondrial mass under developmental and pathophysiological conditions. Cellular BNIP3 and NIX levels are tightly controlled by SCFFBXL4-mediated ubiquitination to prevent excessive mitophagy and lethal disease. Here we report that knockout of PPTC7, a mitochondrial matrix protein, hyperactivates BNIP3/NIX-mediated mitophagy in vitro and in vivo. Biochemically, the PPTC7 precursor is trapped by BNIP3 and NIX to mitochondrial outer-membrane, where PPTC7 scaffolds the assembly of the substrate-PPTC7-SCFFBXL4 holocomplex to degrade BNIP3 and NIX, forming a homeostatic regulatory loop. PPTC7 possesses an unusually weak mitochondrial targeting sequence to facilitate its outer-membrane retention and mitophagy control. Upon starvation, PPTC7 is transcriptionally upregulated in mouse liver to repress mitophagy, which critically maintains hepatic mitochondrial mass, bioenergetics and gluconeogenesis. Collectively, PPTC7 functions as a mitophagy sensor that integrates homeostatic and physiological signals to dynamically control BNIP3 and NIX degradation, thereby maintaining proper mitochondrial mass and cellular homeostasis. |
| Institute: | National Institute of Biological Sciences,Beijing |
| Last Name: | Ma |
| First Name: | Yan |
| Address: | Zhongguancun Life Science Park |
| Email: | mayan@nibs.ac.cn |
| Phone: | +86-01080726688 |
