Summary of Study ST002932
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001823. The data can be accessed directly via it's Project DOI: 10.21228/M82Q61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002932 |
| Study Title | Analyzing Metabolic Alterations in the Gut, Hippocampus and Brainstem of Mice Induced by Running Exercise Through Liquid Chromatography Mass Spectrometry |
| Study Summary | Metabolic analysis of the impact of running exercise on the gut, hippocampus and brainstem is crucial as it provides insights into how exercise affects memory, mood, and vital physiological functions. In our six-week study, we utilized a mouse model (C57BL/6 genotype) to investigate these metabolic changes. Employing liquid chromatography coupled with mass spectrometry followed by metabolomics for a comprehensive analysis, our approach offers insights into how exercise influences metabolic processes, including brain function. Our findings hold the potential to shape more effective exercise strategies for enhancing overall health and cognitive function. |
| Institute | University of Puerto Rico, School of Medicine |
| Department | Biochemistry |
| Last Name | Chorna |
| First Name | Nataliya |
| Address | University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00935 |
| nataliya.chorna@upr.edu | |
| Phone | 7877582525 ext 1640 |
| Submit Date | 2023-10-15 |
| Num Groups | 2 |
| Total Subjects | 24 |
| Num Males | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004810 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Synapt-XS Waters |
| Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Ion Mobility TOF |
| MS instrument name | Waters Synapt-XS |
| Ion Mode | POSITIVE |
| Units | peak intensities |
Chromatography:
| Chromatography ID: | CH003635 |
| Chromatography Summary: | The LC/MS experiments were conducted using an i-Class LC system coupled to a Synapt-XS mass spectrometer (Waters). Liquid chromatography was performed on fecal, hippocampus, and brainstem samples collected from both the SED and RUN groups. The chromatographic separation was conducted using a BEH HILIC column with 1.7 µm particle size dimensions and 2.1 x 100 mm (Waters). The column temperature was maintained at 50ºC, and the flow rate was set at 0.4 mL/min. The mobile phase consisted of 0.1% formic acid in water as solvent A and 0.1% formic acid in acetonitrile as solvent B. The initial mobile phase composition was 5% A and 95% B, which was held constant for 2 minutes. Subsequently, a linear gradient from 5% to 50% A was applied over 8 minutes, followed by a 2-minute hold at 50% B before returning to the initial condition for a 5-minute re-equilibration. The total run time for each analysis was 19 minutes. |
| Instrument Name: | Synapt-XS Waters |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Column Temperature: | 50ºC |
| Flow Gradient: | 5% to 50% A was applied over 8 minutes, followed by a 2-minute hold at 50% B before returning to the initial condition for a 5-minute re-equilibration. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
