Summary of Study ST002932
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001823. The data can be accessed directly via it's Project DOI: 10.21228/M82Q61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002932 |
| Study Title | Analyzing Metabolic Alterations in the Gut, Hippocampus and Brainstem of Mice Induced by Running Exercise Through Liquid Chromatography Mass Spectrometry |
| Study Summary | Metabolic analysis of the impact of running exercise on the gut, hippocampus and brainstem is crucial as it provides insights into how exercise affects memory, mood, and vital physiological functions. In our six-week study, we utilized a mouse model (C57BL/6 genotype) to investigate these metabolic changes. Employing liquid chromatography coupled with mass spectrometry followed by metabolomics for a comprehensive analysis, our approach offers insights into how exercise influences metabolic processes, including brain function. Our findings hold the potential to shape more effective exercise strategies for enhancing overall health and cognitive function. |
| Institute | University of Puerto Rico, School of Medicine |
| Department | Biochemistry |
| Last Name | Chorna |
| First Name | Nataliya |
| Address | University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00935 |
| nataliya.chorna@upr.edu | |
| Phone | 7877582525 ext 1640 |
| Submit Date | 2023-10-15 |
| Num Groups | 2 |
| Total Subjects | 24 |
| Num Males | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-30 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003038 |
| Collection Summary: | Extraction of fecal metabolites: 50 mg of fresh feces were collected from each mouse and frozen in liquid nitrogen. Samples were spiked with 0.5 mL water, homogenized for 5 min followed by 10 s of sonication, and centrifuged at 14K rpm X 10 min at 4°C. Collected supernatants were evaporated to dryness under a nitrogen gas stream and stored at −80°C. Extraction of brain metabolites: Approximately 20-40 mg of frozen tissues was resuspended in 4 volumes of MPER and homogenized on ice. 130 µL of tissue homogenates were spin filtered, as above, and the flow through was diluted 1:1 with deionized water, followed by sonication for 10 sec using a Sonic Dismembrator on ice, vortexed for 10 s, and centrifuged at 14K rpm X 15 min at 4°C. 100 uL of eluate was evaporated to dryness under a nitrogen gas stream and stored at −80°C. |
| Sample Type: | feces, hippocampus, brainstem |
| Storage Conditions: | -80℃ |
