Summary of Study ST003151
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003151 |
| Study Title | BT474 breast cancer cell line grown in 20% D2O-containing RPMI-1640 medium treated with Fasnall and GSK2194069 |
| Study Type | Free fatty acid analysis, D2O tracing |
| Study Summary | BT474 breast cancer cell line grown in 20% D2O-containing medium treated with Fasnall and GSK2194069. Cells were grown for 24 h in RPMI-1640 with 10% dialyzed FBS. |
| Institute | Wistar Institute |
| Department | Molecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center |
| Laboratory | Schug's Lab |
| Last Name | Mukha |
| First Name | Dzmitry |
| Address | 3601 Spruce St, Philadelphia, PA 19104, USA |
| dmukha@wistar.org | |
| Phone | 2154956903 |
| Submit Date | 2024-03-09 |
| Num Groups | 7 |
| Total Subjects | 21 |
| Publications | Submission Pending |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005169 | AN005170 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | Phenomenex Kinetex XB-C18 (150 x 3 mm, 2.6 um) | Phenomenex Kinetex XB-C18 (150 x 3 mm, 2.6 um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | AU | AU |
Chromatography:
| Chromatography ID: | CH003911 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Phenomenex Kinetex XB-C18 (150 x 3 mm, 2.6 um) |
| Column Temperature: | 65 |
| Flow Gradient: | 0-4.5 min, 15-60% B; 4.5-12 min, 60-82% B; 12-12.75 min, 82-95% B; 12.75-16.5 min, 95-100% B; 16.5-22.5 min, 100% B; 22.5-22.6 min, 100-15% B; 22.6-25 min, 15% B |
| Flow Rate: | 0-22.5 min, 0.333 ml/min; 22.5-22.6 min, 0.333-0.5 ml/min; 22.6-24.9 min, 0.5 ml/min; 24.9-25 min, 0.5-0.333 ml/min |
| Injection Temperature: | 20 |
| Solvent A: | 60% Acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% Isopropanol/8% acetonitrile/2% water; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
