Summary of Study ST003151

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003151
Study TitleBT474 breast cancer cell line grown in 20% D2O-containing RPMI-1640 medium treated with Fasnall and GSK2194069
Study TypeFree fatty acid analysis, D2O tracing
Study SummaryBT474 breast cancer cell line grown in 20% D2O-containing medium treated with Fasnall and GSK2194069. Cells were grown for 24 h in RPMI-1640 with 10% dialyzed FBS.
Institute
Wistar Institute
DepartmentMolecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center
LaboratorySchug's Lab
Last NameMukha
First NameDzmitry
Address3601 Spruce St, Philadelphia, PA 19104, USA
Emaildmukha@wistar.org
Phone2154956903
Submit Date2024-03-09
Num Groups7
Total Subjects21
PublicationsSubmission Pending
Raw Data AvailableYes
Raw Data File Type(s)mzXML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-04-02
Release Version1
Dzmitry Mukha Dzmitry Mukha
https://dx.doi.org/10.21228/M8TM76
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005169 AN005170
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Phenomenex Kinetex XB-C18 (150 x 3 mm, 2.6 um) Phenomenex Kinetex XB-C18 (150 x 3 mm, 2.6 um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap Thermo Q Exactive HF-X Orbitrap
Ion Mode NEGATIVE POSITIVE
Units AU AU

MS:

MS ID:MS004904
Analysis ID:AN005169
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The HESI ion source voltage was set to the following parameters: +3,300/-3,500 V, sheath gas 50, auxiliary gas 20, spare gas 0, probe heater 350 °C, capillary temperature 350 °C, S-Lens RF level 80. The mass spectrometer was set to acquire data in polarity-switching mode, one microscan, 60,000 resolution, AGC target 5e6, scan range 130-1950 m/z, IT 200 ms. Data were converted into the mzXML format by ProteoWizard and analyzed in MAVEN. Free cholesterol was measured as a water-loss in-source fragment [M-H2O+H]+.
Ion Mode:NEGATIVE
Capillary Temperature:350
Ion Source Temperature:350
Ion Spray Voltage:3500
Ionization:Both
Source Temperature:350
Automatic Gain Control:5e6
Dataformat:RAW
  
MS ID:MS004905
Analysis ID:AN005170
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The HESI ion source voltage was set to the following parameters: +3,300/-3,500 V, sheath gas 50, auxiliary gas 20, spare gas 0, probe heater 350 °C, capillary temperature 350 °C, S-Lens RF level 80. The mass spectrometer was set to acquire data in polarity-switching mode, one microscan, 60,000 resolution, AGC target 5e6, scan range 130-1950 m/z, IT 200 ms. Data were converted into the mzXML format by ProteoWizard and analyzed in MAVEN. Free cholesterol was measured as a water-loss in-source fragment [M-H2O+H]+.
Ion Mode:POSITIVE
Capillary Temperature:350
Ion Source Temperature:350
Ion Spray Voltage:3300
Ionization:Both
Source Temperature:350
Automatic Gain Control:5e6
Dataformat:RAW
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