Summary of Study ST000460
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000460 |
| Study Title | Effect of ATG7 reconstitution in AGY571 cells |
| Study Type | Cells are treated with vehicle or Doxycycline - to induce expression of GFP or ATG7 |
| Study Summary | AGY571 cells engineered to express Dox-inducible GFP or ATG7 were treated with 500 ng/mL or 1 ug/mL Doxycycline for 48 hours. Assessing the effects of ATG7 reexpression in B cell lymphoma. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | John Cleveland |
| Last Name | Fernandez |
| First Name | Mario |
| Address | 12902 Magnolia Drive, MRC 4-West |
| mario.fernandez@moffitt.org | |
| Phone | 813-745-5140 |
| Submit Date | 2016-09-08 |
| Num Groups | 5 |
| Total Subjects | 15 |
| Study Comments | Groups collected in triplicate per time point, either treated or untreated with Doxycycline. |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-07-08 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000475 |
| Collection Summary: | Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C. |
| Sample Type: | Cell |
| Collection Method: | Pellet |
| Collection Location: | Lab |
| Collection Frequency: | 48 hours after Dox treatment |
| Storage Conditions: | Pellet at -80C |
| Storage Vials: | 1.5 mL microfuge tube |
| Tissue Cell Identification: | AGY571 cells |
