Summary of Study ST000961

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000659. The data can be accessed directly via it's Project DOI: 10.21228/M8GX0J This work is supported by NIH grant, U2C- DK119886.


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Study IDST000961
Study TitleThe Influence of Sugar, Artificial Sweeteners, and the Microbiome on Rodent Amino Acids (part IV)
Study SummaryTargeted Amino Acid panel of rodents treated with diets rich in commonly used artifically sweeteners will be assessed in this study. We hypothesized that a specific subset of plasma metabolites are generated as a result from a diet rich in commonly used artificial sweeteners and their subsequent processing by the gut microbiome, which could ultimately lead to impaired glycemic control and negative physiological health outcomes. To test this hypothesis we administered normal, high glucose, fructose, aspartame, and acesulfame potassium diets to rats for 3 weeks, followed by a plasma collection through cardiac puncture and metabolic analysis. We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals. The resulting data will give us insights into the influence of high sugar and artificial sweetener diets on homeostatic metabolic processes and dive into the symbiotic relationship of the gut microbiome with this process.
Mayo Clinic
Last NameHoffmann
First NameBrian
Address8701 Watertown Plank Road Milwaukee, WI 53226
Submit Date2018-04-14
Analysis Type DetailLC-MS
Release Date2020-04-15
Release Version1
Brian Hoffmann Brian Hoffmann application/zip

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Collection ID:CO000994
Collection Summary:Samples were collected by cardiac puncture and plasma was collected following standard centrifugation steps. Immediately following the samples were separated into 500 uL aliquots in 600 uL tubes and frozen in liquid nitrogen.
Sample Type:Blood (plasma)