Summary of Study ST001095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000733. The data can be accessed directly via it's Project DOI: 10.21228/M8XM40 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001095 |
| Study Title | Data resource for fully 13C labelled and non-labelled plant tissues (part-I) |
| Study Summary | LC-MS/MS data files of fully 13C labelled and 12C plant tissues, which were utilized to determine the carbon element number for metabolite characterizations. |
| Institute | RIKEN |
| Department | Center for Sustainable Resource Science |
| Laboratory | Metabolome Informatics Research Team |
| Last Name | Tsugawa |
| First Name | Hiroshi |
| Address | 1-7-22 Suehiro-cho, Tsurumi-ku |
| hiroshi.tsugawa@riken.jp | |
| Phone | +81-45-503-9618 |
| Submit Date | 2018-11-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001133 |
| Collection Summary: | Leaf/stem and root tissues of Arabidopsis thaliana, Nicotiana tabacum, Glycine max, Glycyrrhiza glabra, Glycyrrhiza urahensis, and Oryza sativa, bulb, leaf, and root tissues of Allium cepa, flower, leaf/stem, seed pod, seed, and root tissues of Medicago truncatula, green and ripe fruit-, and leaf/stem tissues of Solanum lycopersicum, leaf/stem, root, and tuber tissues of Solanum tuberosum, and leaf, stem, seed, and root tissues of Zea mays were purchased from IsoLife Co. (Wageningen UR, Netherlands). The hairy root of Ophiorrhiza pumila was grown as follows. O. pumila hairy roots were derived and maintained, except for the carbon source. We supplied 1.5% D-glucose instead of 1.5% sucrose. We obtained 4-week-old O. pumila hairy roots and sub-cultured them in 1.5% D-glucose (U-13C6, 99%) (Cambridge Isotope Laboratories, Inc.) for two generations for complete isotope labeling of the metabolome. Hairy roots from the same batch used for isotope-labeling were sub-cultured for two subcultures in 1.5% D-glucose (Nacalai Tesque, Inc); they served as the control. Hairy roots from the second sub-culture of sub-cultured tissues growing in 1.5% isotope-labelled or un-labelled 1.5% D-glucose were harvested, wiped with paper towels and snap-frozen in liquid-N2. |
| Sample Type: | Plant |
