Summary of Study ST001224
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000821. The data can be accessed directly via it's Project DOI: 10.21228/M8JX05 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001224 |
| Study Title | Vaginal swab lipidome profiles at 48 h reflect the fat composition of neonatal diet during first two days postnatal |
| Study Type | MRM-profiling |
| Study Summary | In this study, we further investigated the efficacy of using MRM-profiling of vaginal lipids to differentiate PND 2 vaginal swabs between gilts suckled by sow or fed milk replacer. Secondly, we tested the effect of a lard based supplement on vaginal lipid profiles of gilts. |
| Institute | Purdue University |
| Department | Animal Sciences |
| Laboratory | Metabolite Profiling Facility - Purdue University |
| Last Name | Ferreira |
| First Name | Christina |
| Address | 1203 W. State St, West Lafayette, IN, 47906, USA |
| cferrei@purdue.edu | |
| Phone | 7654095924 |
| Submit Date | 2019-06-06 |
| Num Groups | colostrum suckled (S, n=8); S plus fat supplement (SF, n=5); bottle-fed milk replacer (B, n=8); or B plus fat supplement (BF, n=7 |
| Total Subjects | 28 |
| Num Females | 28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001285 |
| Collection Summary: | Blood samples were collected from gilts at 48 h postnatal. Gilts were euthanized approximately 48 h after birth using CO2 inhalation. Following euthanasia, skin of the abdominal and genital regions was cleaned thoroughly using 70% ethanol, and vaginal swabs were taken using a cytology brush (Puritan 2196 Removable Stiff Bristle Tip Brush; QuickMedical; Issaquah, WA) by inserting the tip of the brush into the vulva angled dorsally at 45˚. Once inserted to the base of the bristles, the brush was rotated 360˚ against the vaginal surface. Two consecutive swabs were collected from each animal, and swabs were placed in separate 15 ml sterile polypropylene conical tubes (Falcon™, Fisher Scientific, San Jose, California) and immediately placed on dry ice for transport. Samples were stored in a -80°C freezer until lipid extraction and analysis. Sows were milked during farrowing, and at 24 h after delivery of first piglet. For milk collection piglets were removed from the sow for approximately an hour and then 1 ml oxytocin (VetOne; Boise, ID; 20 USP/ml) was administered IM using a 20g x 1.5-inch needle into the vulva to stimulate milk letdown. Colostrum samples were collected manually from all teats and combined to create a uniform sample. Samples were stored until further analysis at -20°C |
| Sample Type: | Vaginal epithelium |
| Collection Method: | swab |
| Collection Location: | West Lafayette |
| Collection Frequency: | once |
| Collection Duration: | 1min |
| Storage Conditions: | -80℃ |
| Collection Vials: | Puritan 2196 Removable Stiff Bristle Tip Brush; QuickMedical; Issaquah, WA |
| Storage Vials: | 15 ml sterile polypropylene conical tubes (Falcon™, Fisher Scientific, San Jose, California) |
| Additives: | none |
