Summary of Study ST001224
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000821. The data can be accessed directly via it's Project DOI: 10.21228/M8JX05 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001224 |
| Study Title | Vaginal swab lipidome profiles at 48 h reflect the fat composition of neonatal diet during first two days postnatal |
| Study Type | MRM-profiling |
| Study Summary | In this study, we further investigated the efficacy of using MRM-profiling of vaginal lipids to differentiate PND 2 vaginal swabs between gilts suckled by sow or fed milk replacer. Secondly, we tested the effect of a lard based supplement on vaginal lipid profiles of gilts. |
| Institute | Purdue University |
| Department | Animal Sciences |
| Laboratory | Metabolite Profiling Facility - Purdue University |
| Last Name | Ferreira |
| First Name | Christina |
| Address | 1203 W. State St, West Lafayette, IN, 47906, USA |
| cferrei@purdue.edu | |
| Phone | 7654095924 |
| Submit Date | 2019-06-06 |
| Num Groups | colostrum suckled (S, n=8); S plus fat supplement (SF, n=5); bottle-fed milk replacer (B, n=8); or B plus fat supplement (BF, n=7 |
| Total Subjects | 28 |
| Num Females | 28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001299 |
| Sampleprep Summary: | The Bligh & Dyer lipid extraction technique was slightly modified to extract lipids from the vaginal swab samples. A volume of 500 µl distilled water was added to the conical tube containing the swab brushes and vortexed to remove biological material from the brush. The brushes were removed, the sample homogenate was transferred to a new tube, and phase separation was performed by mixing with chloroform/methanol/distilled water (1:2:0.8). Samples were centrifuged, the organic phase (bottom phase) was separated from aqueous phase, divided into four aliquots, and dried in a centrifugal evaporator (Savant SpeedVac AES2010, ThermoFisher Scientific, San Jose, CA). Dried lipid extracts were stored at -20˚C until mass spectrometry analysis. The Bligh & Dyer lipid extraction technique was also used to extract lipids from 48 h serum samples from suckled and bottle-fed piglets; colostrum samples taken from all eight sows (during parturition, 6 h after first piglet born, 12 h after, and 24 h after); and milk replacer used for bottle-feeding. The procedure for these samples began at the phase separation step (i.e. no water was added to the samples). |
| Processing Storage Conditions: | Room temperature |
| Extraction Method: | Bligh & Dyer |
| Extract Storage: | -80℃ |
| Sample Resuspension: | acetonitrile/methanol/ammonium acetate 300 mM at 3:6.65:0.35 (v/v) |
| Sample Derivatization: | none |
| Sample Spiking: | none |
