Summary of study ST001320

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000897. The data can be accessed directly via it's Project DOI: 10.21228/M8R704 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001320
Study TitleExamination of labeled glucose utilization in central carbon metabolism in HeLa cells post WNT stimulation
Study TypeIn vitro
Study SummaryHeLa cells were treated with media containing either vehicle or WNT in combination with UC13-glucose for 20 min or 60 min.
Institute
University of California, Los Angeles
DepartmentBiological Chemisty
LaboratoryChristofk Lab
Last NameChristofk
First NameHeather
Address615 Charles E Young Drive South Los Angeles, CA 90095
Emailhchristofk@mednet.ucla.edu
Phone(310) 794-4248
Submit Date2020-02-19
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2020-07-18
Release Version1
Heather Christofk Heather Christofk
https://dx.doi.org/10.21228/M8R704
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001389
Collection Summary:Extraction began by placing the 6-well dishes on ice and washing each well with 2mL of a 150 mM ammonium acetate solution (pH 7.4, 4°C). Post wash, metabolites were extracted from each well on dry ice with 500 μL of a (1:4) dH20, MeOH solution at -80°C. The samples were then incubated for 15 min at -80°C. Afterwards, the cells were scraped into solutions which were then transferred to eppitubes and spun down at 4°C for 10 min at 17,000g. The supernatants were transferred to new glass vials and dried with an EZ2-Elite lyophilizer (Genevac). These dried metabolites were then stored at -80°C until they could be run on the LC/MS.
Sample Type:Cultured cells
Storage Conditions:Described in summary
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