Summary of study ST001437

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000988. The data can be accessed directly via it's Project DOI: 10.21228/M80680 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001437
Study TitleSub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - EBC
Study SummaryThe human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. The first set of samples tested for this approach included exhaled breath condensates (EBC) collected from cystic fibrosis (CF) patients with impaired glucose tolerance to study the metabolome changes before and after the oral glucose tolerance test.
Institute
Georgia Institute of Technology
Last NameFacundo
First NameFernandez
Address901 Atlantic Dr NW
Emailfernandez@gatech.edu
Phone(404) 385-4432
Submit Date2020-07-29
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailESI
Release Date2020-09-14
Release Version1
Fernandez Facundo Fernandez Facundo
https://dx.doi.org/10.21228/M80680
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001506
Collection Summary:EBC from 11 CF patients with abnormalities in glucose homeostasis (as defined by having prediabetes/impaired glucose tolerance [CF IGT] on an oral glucose tolerance test) was collected both fasting and 2 hours following ingestion of a glucose drink. They were termed as “Pre” and “Post”, respectively. Of these 11 subjects, one did not have an adequate sample for the “Pre” and another did not have a sample for the “Post” study giving a total of 20 samples analyzed (one of then failed to purduce enough features as others and was regarded as an outlier and removed). EBC sample collection followed the guidelines approved by the Georgia Institute of Technology and the Emory University Institutional Review Boards (approval number IRB00000372). Samples were collected with an R-Tube collector (Respiratory Research, Inc., Austin, TX, USA). After collection, samples were immediately frozen at -80℃ until processed.
Sample Type:Exhaled Breath condensate
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