Summary of study ST001496

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001014. The data can be accessed directly via it's Project DOI: 10.21228/M8MT3W This work is supported by NIH grant, U2C- DK119886.

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Study IDST001496
Study TitleApplication of developed quantatation method
Study SummaryTo apply this method in a pilot study, we spiked either taurine or sulfoquinovose into an in vitro simplified human microbiota model with and without Bilophila wadsworthia, a known sulfonate utilizer.
Institute
Helmholtz Centre for Environmental Research - UFZ
Last NameHaange
First NameSven
AddressPermoserstrasse 1, Leipzig, Saxony, 04318, Germany
Emailsven.haange@ufz.de
Phone0049 341 2351099
Submit Date2020-10-02
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2020-10-28
Release Version1
Sven Haange Sven Haange
https://dx.doi.org/10.21228/M8MT3W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001565
Collection Summary:For each 10 ml incubation, DS medium supplemented with either taurine (20 mM) or sulfoquinovose (SQ, 4 mM) was inoculated with the SIHUMI bacteria or the SIHUMI consortium and B. wadsworthia. Control incubations of medium containing only sulfonates or bacteria were included. The incubations were conducted under anoxic conditions at 37 °C in duplicates. Samples (600 µl) were withdrawn after 0, 3, 24 and 48 h for quantification of sulfoquinovose (SQ), 2,3-dihydroxypropane-1-sulfonate (DHPS), taurine and isethionate.
Sample Type:Bacterial cells
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