Summary of Study ST001779

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001132. The data can be accessed directly via it's Project DOI: 10.21228/M8CQ5F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001779
Study TitleUntargeted Metabolomics analysis of A549 treated with 0.5 mM extracellular ATP and 10 ng/ml TGF-beta
Study TypeUntargeted metabolomics analysis in lung cancer cells
Study SummaryControl, 0.5 mM extracellular ATP and 10 ng/ml TGF-beta were used to treated 5 million A549 lung cancer cells in vitro for 2, 6 and 12 hours. The untargeted metabolomics analysis was performed on the cell lysates. The main objective of the study was to determine changes in metabolite abundances in lung cancer after treatment with extracellular ATP and TGF-beta (a known EMT inducer).
Institute
Ohio University
DepartmentBiological Sciences
LaboratoryDr. Xiaozhuo Chen, Edison biotechnology Institute
Last NameShriwas
First NamePratik
AddressRoom 425, Parks Hall, College of Pharmacy, Ohio State University, Columbus Ohio. 43210
Emailps774614@ohio.edu
Phone7406033801
Submit Date2021-04-04
Num Groups7
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2021-05-21
Release Version1
Pratik Shriwas Pratik Shriwas
https://dx.doi.org/10.21228/M8CQ5F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001849
Collection Summary:A549 lung cancer cells were treated with control, ATP, TGF-beta and then collected according to sample preparation protocol.
Collection Protocol Filename:Collection protocol
Sample Type:Lung
Collection Method:Ice cold methanol (80%) using cell scrappers
Collection Location:150mm dish
Collection Frequency:Once
Collection Duration:1-2 sec
Volumeoramount Collected:1 ml
Storage Conditions:-80℃
Collection Vials:Polypropylene 1.5 ml tubes
Storage Vials:Polypropylene 1.5 ml
Collection Tube Temp:4C
Tissue Cell Quantity Taken:5 million
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