Summary of Study ST001779
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001132. The data can be accessed directly via it's Project DOI: 10.21228/M8CQ5F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001779 |
| Study Title | Untargeted Metabolomics analysis of A549 treated with 0.5 mM extracellular ATP and 10 ng/ml TGF-beta |
| Study Type | Untargeted metabolomics analysis in lung cancer cells |
| Study Summary | Control, 0.5 mM extracellular ATP and 10 ng/ml TGF-beta were used to treated 5 million A549 lung cancer cells in vitro for 2, 6 and 12 hours. The untargeted metabolomics analysis was performed on the cell lysates. The main objective of the study was to determine changes in metabolite abundances in lung cancer after treatment with extracellular ATP and TGF-beta (a known EMT inducer). |
| Institute | Ohio University |
| Department | Biological Sciences |
| Laboratory | Dr. Xiaozhuo Chen, Edison biotechnology Institute |
| Last Name | Shriwas |
| First Name | Pratik |
| Address | Room 425, Parks Hall, College of Pharmacy, Ohio State University, Columbus Ohio. 43210 |
| ps774614@ohio.edu | |
| Phone | 7406033801 |
| Submit Date | 2021-04-04 |
| Num Groups | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001862 |
| Sampleprep Summary: | 5 × 106 A549 cells were treated with control (no treatment), 0.5 mM ATP or 10 ng/ml TGF-beta for 2, 6 and 12 hours. After treatment, cells were washed twice with deionized water and polar metabolites were then extracted with cryogenically cold 80% methanol/water mixture. LC-MS grade water, methanol, and acetonitrile (Fischer Scientific, PA, USA) were used. Methanol-extracted samples were then sonicated in cycles of sonication phase and rest phase for 10 minutes (5 second sonication phase and 10 seconds halt). The samples were then centrifuged at 13,000 rpm for 10 minutes and supernatant was then collected. Supernatants collected from in vitro and in vivo extraction were then lyophilized. Briefly, the supernatant was then lyophilized by using a speed vacuum evaporator. The samples were then dissolved into a mixture of acetonitrile/water (1:1; v/v). |
| Sampleprep Protocol Filename: | Sample preparation protocol |
| Processing Method: | CEll scrapping Quenching |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Quenching with Ice cold methanol |
| Extract Enrichment: | Speed vaccum evaporator |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Acetonitrile/water (1:1) |
