Summary of Study ST002184

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001391. The data can be accessed directly via it's Project DOI: 10.21228/M8X99S This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002184
Study TitleMetabolic effect of the loss of mitochondrial-specific aspartyl-tRNA synthetase Das2 on mouse intestinal epithelial cells
Study SummaryWe analysed the intestinal epithelial cell (IEC) from Dars2 fl/fl ; VillinCreERT2 tg/wt mice (n=15) and and Dars2 fl/fl ; VillinCreERT2 wt/wt mice (n=9) at 8 days upon tamoxifen injection to assess the metabolic effect of Das2 loss. Isolated IECs were divided into three technical replicates (n=69) and analysed with two analytical repeats (n=138).
Institute
CECAD Research Center
Last NameYang
First NameMing
AddressJoseph-Stelzmann-Straße 26, Köln, Koeln, 50931, Germany
Emailming.yang@uni-koeln.de
Phone4922147884306
Submit Date2022-06-01
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-06-15
Release Version1
Ming Yang Ming Yang
https://dx.doi.org/10.21228/M8X99S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002263
Collection Summary:Small intestines were dissected and washed with PBS. Small pieces of approximately ~0.5 cm were isolated from proximal (post stomach) small intestine and snap frozen on dry ice and stored at -80°C until further processing. The remaining small intestinal tissue was washed in PBS to remove faeces and cut longitudinally. Intestinal epithelial cells were isolated by sequential incubation of intestinal tissue in pre-heated 1 mM dithiothreitol and 1.5 mM EDTA solutions at 37oC while shaking. Intestinal epithelial cell pellet was frozen at -80°C until further processing. Intestinal epithelial cell (IEC) pellet was frozen at -80oC for further processing.
Sample Type:Intestine
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