Summary of Study ST002184

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001391. The data can be accessed directly via it's Project DOI: 10.21228/M8X99S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002184
Study Title	Metabolic effect of the loss of mitochondrial-specific aspartyl-tRNA synthetase Das2 on mouse intestinal epithelial cells
Study Summary	We analysed the intestinal epithelial cell (IEC) from Dars2 fl/fl ; VillinCreERT2 tg/wt mice (n=15) and and Dars2 fl/fl ; VillinCreERT2 wt/wt mice (n=9) at 8 days upon tamoxifen injection to assess the metabolic effect of Das2 loss. Isolated IECs were divided into three technical replicates (n=69) and analysed with two analytical repeats (n=138).
Institute	CECAD Research Center
Last Name	Yang
First Name	Ming
Address	Joseph-Stelzmann-Straße 26, Köln, Koeln, 50931, Germany
Email	ming.yang@uni-koeln.de
Phone	4922147884306
Submit Date	2022-06-01
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2022-06-15
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002276
Sampleprep Summary:	Metabolite extraction solution (50% methanol, 30% acetonitrile, 20% water, 5uM valine-d8 as internal standard) was added to (10-20mg) frozen small intestine tissue samples at an extraction ratio of 25ul/mg on dry ice. Samples were then homogenized using a Precellys 24 tissue homogenizer (Bertin technologies). The resulting sample suspension was vortexed, mixed at 4oC in a Thermomixer for 15 min at 1,500 rpm and then centrifuged at 16,000 x g for 20 min at 4oC. The supernatant was collected for LC-MS analysis.

Sampleprep ID:

SP002276

Sampleprep Summary:

Metabolite extraction solution (50% methanol, 30% acetonitrile, 20% water, 5uM valine-d8 as internal standard) was added to (10-20mg) frozen small intestine tissue samples at an extraction ratio of 25ul/mg on dry ice. Samples were then homogenized using a Precellys 24 tissue homogenizer (Bertin technologies). The resulting sample suspension was vortexed, mixed at 4oC in a Thermomixer for 15 min at 1,500 rpm and then centrifuged at 16,000 x g for 20 min at 4oC. The supernatant was collected for LC-MS analysis.