Summary of Study ST002208
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001410. The data can be accessed directly via it's Project DOI: 10.21228/M8G42C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002208 |
| Study Title | Metabolomic analysis to assess response to immunotherapy for malignant brain tumors: Part 2 |
| Study Type | Cancer surrogate biomarker discovery |
| Study Summary | An effective immune response in patients with cancer treated with immunotherapy includes dendritic cell (DC) activation and migration followed by stimulation of CD8 and CD4 T cells. This then leads to the activation, proliferation and further activation of other immune cell populations including NK cells or immunosuppressive populations such as Tregs and myeloid derived suppressor cells (MDSCs). These studies were carried out utilizing murine brain tumor models treated with an RNA DC vaccine platform. We hypothesized that metabolomic analyses of urines would be sensitive to the action of this diverse set of immune cells. The objective of this study was to evaluate the feasibility of using metabolomics to follow immune responses after immunotherapy. We chose NMR as our analytical technique of choice, as it has many favorable qualities that make it ideal for analyses of urine. |
| Institute | University of Florida |
| Department | Applied Physiology and Kinesiology |
| Laboratory | Rm 042 |
| Last Name | Khattri |
| First Name | Ram |
| Address | 1200 Newell Dr., ARB 240, Gainesville, FL, 32611, USA |
| rbk11@ufl.edu | |
| Phone | 3307856045 |
| Submit Date | 2022-06-09 |
| Num Groups | 10 |
| Total Subjects | 80 |
| Num Males | NA |
| Num Females | NA |
| Study Comments | Metabolomic profiling of urine samples |
| Publications | Metabolomics journal (submitted) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-06-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO002287 |
| Collection Summary: | Intakt urine samples were collected from C57/Bl6 mice. Urine is collected from a clean surface after urination. |
| Sample Type: | Urine |
| Collection Method: | Each animal was in a separate chamber individually. The chamber bottom was covered with clean parafilm. Animals were kept in the chamber until urination. Urine drops were collected using sterile syringes. Samples were stored at -80 °C until further analysis. |
| Collection Location: | University of Florida, Neurosurgery Department, Medical college, University of Florida |
| Collection Frequency: | Post-vaccination day 1, day 8, day 12, day 16, and day 20 |
| Collection Duration: | ~30 minutes |
| Volumeoramount Collected: | ~100 uL |
| Storage Conditions: | -80℃ |
| Collection Vials: | cryovials |
| Storage Vials: | cryovials |
