Summary of Study ST002208

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001410. The data can be accessed directly via it's Project DOI: 10.21228/M8G42C This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002208
Study TitleMetabolomic analysis to assess response to immunotherapy for malignant brain tumors: Part 2
Study TypeCancer surrogate biomarker discovery
Study SummaryAn effective immune response in patients with cancer treated with immunotherapy includes dendritic cell (DC) activation and migration followed by stimulation of CD8 and CD4 T cells. This then leads to the activation, proliferation and further activation of other immune cell populations including NK cells or immunosuppressive populations such as Tregs and myeloid derived suppressor cells (MDSCs). These studies were carried out utilizing murine brain tumor models treated with an RNA DC vaccine platform. We hypothesized that metabolomic analyses of urines would be sensitive to the action of this diverse set of immune cells. The objective of this study was to evaluate the feasibility of using metabolomics to follow immune responses after immunotherapy. We chose NMR as our analytical technique of choice, as it has many favorable qualities that make it ideal for analyses of urine.
University of Florida
DepartmentApplied Physiology and Kinesiology
LaboratoryRm 042
Last NameKhattri
First NameRam
Address1200 Newell Dr., ARB 240, Gainesville, FL, 32611, USA
Submit Date2022-06-09
Num Groups10
Total Subjects80
Num MalesNA
Num FemalesNA
Study CommentsMetabolomic profiling of urine samples
PublicationsMetabolomics journal (submitted)
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2023-06-16
Release Version1
Ram Khattri Ram Khattri application/zip

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Sample Preparation:

Sampleprep ID:SP002300
Sampleprep Summary:No filtration was performed for urine samples. The urine samples were centrifuged at 4 oC, with 13.2 K rpm speed before mixing it with an internal reference and phosphate buffer system.
Sampleprep Protocol Filename:DC_Vaccine_treated_NMR_urine_Procedures.docx
Processing Method:None
Processing Storage Conditions:-80℃
Extraction Method:None
Extract Storage:-80℃
Sample Resuspension:In 500 microliter of 150 mM phosphate buffer (pH 7.2) with 2 mM EDTA, 0.5 mM DSS and 0.2% sodium azide for aqueous phase samples.
Sample Spiking:0.5 mM DSS for urine samples