Summary of Study ST002389

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001536. The data can be accessed directly via it's Project DOI: 10.21228/M8512J This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002389
Study TitleAlterations in SHH signal transduction introduce a state of hypometabolism in sporadic Parkinson's disease
Study SummaryInduced pluripotent stem cells (iPSC) derived from sporadic Parkinson's disease patients and healthy control subjects were used for disease modeling. iPSC were differentiated towards midbrain dopaminergic neurons. For metabolic analysis, midbrain neuronal precursor cells were cultivated in growth medium supplemented with either 1.25 mM [U-13C]-glutamine or 21.25 mM [U-13C]-glucose. Metabolites were extracted and analyzed using GC-MS. The MetaboliteDetector software was used to analyze chromatograms, calculate mass isotopomer distributions (MIDs) and perform relative comparison of metabolite levels.
Institute
Helmholtz Centre for Environmental Research
DepartmentInstitute of Developmental Genetics
Last NameSchmidt
First NameSebastian
AddressIngolstädter Landstraße 1, 85764 Munich, Germany
Emailsebastian.schmidt@helmholtz-muenchen.de
Phone+4989318743660
Submit Date2022-12-03
Num Groups3
Total Subjects13
Raw Data AvailableYes
Raw Data File Type(s)bin
Analysis Type DetailGC-MS
Release Date2023-10-06
Release Version1
Sebastian Schmidt Sebastian Schmidt
https://dx.doi.org/10.21228/M8512J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002471
Collection Summary:Neuronal precursor cells differentiated from patient derived induced pluripotent stem cells were seeded on Geltrex coated 6-well plates containing neuronal precursor maintenance medium at a density of 1,000,000 cells/well with six replicates per cell line. hNPCs were cultured at 37 °C, 7 % CO2, 21 % O2 for at least 72 h.
Sample Type:Neurons
Storage Conditions:-80?
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