Summary of Study ST002389
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001536. The data can be accessed directly via it's Project DOI: 10.21228/M8512J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002389 |
Study Title | Alterations in SHH signal transduction introduce a state of hypometabolism in sporadic Parkinson's disease |
Study Summary | Induced pluripotent stem cells (iPSC) derived from sporadic Parkinson's disease patients and healthy control subjects were used for disease modeling. iPSC were differentiated towards midbrain dopaminergic neurons. For metabolic analysis, midbrain neuronal precursor cells were cultivated in growth medium supplemented with either 1.25 mM [U-13C]-glutamine or 21.25 mM [U-13C]-glucose. Metabolites were extracted and analyzed using GC-MS. The MetaboliteDetector software was used to analyze chromatograms, calculate mass isotopomer distributions (MIDs) and perform relative comparison of metabolite levels. |
Institute | Helmholtz Centre for Environmental Research |
Department | Institute of Developmental Genetics |
Last Name | Schmidt |
First Name | Sebastian |
Address | Ingolstädter Landstraße 1, 85764 Munich, Germany |
sebastian.schmidt@helmholtz-muenchen.de | |
Phone | +4989318743660 |
Submit Date | 2022-12-03 |
Num Groups | 3 |
Total Subjects | 13 |
Raw Data Available | Yes |
Raw Data File Type(s) | bin |
Analysis Type Detail | GC-MS |
Release Date | 2023-10-06 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002484 |
Sampleprep Summary: | Subsequently, cell culture supernatant was stored for profiling the extracellular metabolome. Three replicates per cell line and blanks were washed with 0.9% NaCl and quenched with ice-cold methanol and ice-cold ddH2O (containing 1 µg/ml D6-glutaric acid as internal standard). Cells were scraped and extracts were added into tubes containing ice-cold chloroform. Following vortexing at 1,400 rpm for 20 min at 4°C and centrifugation at 17,000 g for 5 min at 4°C, 300 µl of the polar phase were transferred into GC glass vials with microinsert and dried under vacuum at 4°C. Dried extracts were derivatized using equal amounts of methoxylamine (20 mg/ml in pyridine) and MTBSTFA. |