Summary of Study ST002413
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001553. The data can be accessed directly via it's Project DOI: 10.21228/M8ZB01 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002413 |
| Study Title | IFN-inducible phospholipid levels govern endosomal antiviral immunity |
| Study Summary | Untargeted lipidomics of 3 cell lines at baseline with focus on phospholipids to understand the role of these lipids in endosomal antiviral immunity |
| Institute | University of Colorado Denver |
| Laboratory | Angelo D'Alessandro |
| Last Name | Haines |
| First Name | Julie |
| Address | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| julie.haines@cuanschutz.edu | |
| Phone | 3037243339 |
| Submit Date | 2022-12-16 |
| Num Groups | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002495 |
| Collection Summary: | The human embryonic kidney 293T (HEK293T) and K562 cells were maintained in Iscove's modified Dulbecco’s medium (IMDM; Sigma), human THP1 cells were maintained in Roswell Park Memorial Institute medium (RPMI; Lonza). All media were supplemented with 10% fetal bovine serum (FBS; Gibco), penicillin (100 IU/ml), streptomycin (100 µg/ml) and 2% glutamine. A549 and Vero E6 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% Non-essential amino acid (NEAA, Gibco), 1% Sodium Pyruvate (Gibco). Calu3 were cultured in Dulbecco's Modified Eagle Medium (DMEM, Sigma) supplemented with 20% fetal bovine serum (FBS; Gibco), 1% Non-essential amino acid (NEAA, Gibco), 1% Sodium Pyruvate (Gibco) and 2% GlutaMAX (Gibco). All cells were maintained in a 5% CO2 humidified atmosphere at 37°C. A549-hACE2 cell lines were generated by lentiviral transduction. Lentiviral vectors were produced following a standard procedure based on calcium phosphate co-transfection with 3rd generation helper and transfer plasmids. Primary cells Human CD34+ HSPC were isolated through positive magnetic bead selection according to manufacturer’s instructions (Milteney) from umbilical cord blood or from mononuclear cells collected upon informed consent from healthy volunteers according to the Institutional Ethical Committee approved protocol (TIGET01). Otherwise, CB, bone marrow (BM) or G-CSF mPB-CD34+ cells were directly purchased from Lonza or Hemacare. All cells were maintained in a 5% CO2 humidified atmosphere at 37°C. |
| Sample Type: | Cultured cells |
