Summary of Study ST002413
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001553. The data can be accessed directly via it's Project DOI: 10.21228/M8ZB01 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002413 |
| Study Title | IFN-inducible phospholipid levels govern endosomal antiviral immunity |
| Study Summary | Untargeted lipidomics of 3 cell lines at baseline with focus on phospholipids to understand the role of these lipids in endosomal antiviral immunity |
| Institute | University of Colorado Denver |
| Laboratory | Angelo D'Alessandro |
| Last Name | Haines |
| First Name | Julie |
| Address | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| julie.haines@cuanschutz.edu | |
| Phone | 3037243339 |
| Submit Date | 2022-12-16 |
| Num Groups | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003933 | AN003934 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
MS:
| MS ID: | MS003671 |
| Analysis ID: | AN003933 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | the mass spectrometer operated in top 10 data dependent MS2 mode with NCE of 30 eV (HCD) and dynamic exclusion of 10 s. MS1 scans were acquired at a resolution of 70,000 across the m/z range of 125-1500. MS2 scans were acquired at a resolution of 17,500. Sheath and auxiliary gases (both N2) were set at 45 and 15 (unitless), respectively. Instrument stability and quality control were assessed via injection of technical replicates every 6 runs. Untargeted lipid results were obtained using LipidSearch (Thermo) with precursor ion tolerance set to 5 ppm and product ion tolerance at 8 ppm. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003672 |
| Analysis ID: | AN003934 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | the mass spectrometer operated in top 10 data dependent MS2 mode with NCE of 30 eV (HCD) and dynamic exclusion of 10 s. MS1 scans were acquired at a resolution of 70,000 across the m/z range of 125-1500. MS2 scans were acquired at a resolution of 17,500. Sheath and auxiliary gases (both N2) were set at 45 and 15 (unitless), respectively. Instrument stability and quality control were assessed via injection of technical replicates every 6 runs. Untargeted lipid results were obtained using LipidSearch (Thermo) with precursor ion tolerance set to 5 ppm and product ion tolerance at 8 ppm. |
| Ion Mode: | POSITIVE |
