Summary of Study ST002526

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002526
Study TitleStable isotope tracing of 15N2-glutamine in orthotopic pancreatic ductal adenocarcinoma tumor bearing mice and non tumor-bearing controls
Study SummaryStable isotope tracing by bolus intravenous injections of 15N2-glutamine in orthotopic PDAC tumor bearing mice and non tumor-bearing controls followed by plasma sampling and tumor extraction for analysis of intratumoral metabolite labeling during the period of kinetic labeling
Institute
University of Chicago
Last NameApiz Saab
First NameJuan
Address929 E. 57th St.
Emailjapizsaab@uchicago.edu
Phone7738346506
Submit Date2022-08-05
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-04-13
Release Version1
Juan Apiz Saab Juan Apiz Saab
https://dx.doi.org/10.21228/M8D434
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002619
Collection Summary:~100uL of blood were be obtained by submandibular sampling as described previously (Parasuraman et al., 2010) and centrifuged at 845 x g for 10 minutes at 4°C to separate plasma. Plasma was frozen in liquid nitrogen and stored at -80°C until further analysis animals. Mice were then immediately euthanized and tumor or pancreas from each animal was then harvested and immediately snap frozen using a BioSqueezer (BioSpec) cooled with liquid nitrogen and stored at -80°F until further analysis.
Sample Type:Tumor, Pancreas & Plasma
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