Summary of Study ST002531

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002531
Study TitleCellular consumption/release of polar metabolites by mPDAC cells cultured in different culture media conditions
Study SummaryWe used quantitative LC-MS metabolite profiling to perform analysis of 108 metabolites that mPDAC cells consume or release while growing in RPMI or TIFM media.
Institute
University of Chicago
Last NameApiz Saab
First NameJuan
Address929 E. 57th St.
Emailjapizsaab@uchicago.edu
Phone7738346506
Submit Date2023-03-24
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-04-13
Release Version1
Juan Apiz Saab Juan Apiz Saab
https://dx.doi.org/10.21228/M8D434
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002624
Collection Summary:100,000-150,000 cells were seeded in 2mL of culture medium in six-well plates with 3 technical replicates per condition per time point and allowed to attach overnight. The following day (day 1), cells were washed twice with 2mL PBS. They were then given 2mL of media, either TIFM or RPMI. An unspent media sample was collected at this time as well and stored at -80 °C. Cell number on day 1 was measured using a Vi-CELL XR Cell Viability Analyzer (Beckman Coulter). 24h later (day 2), 1mL of spent media from cells was collected, centrifuged and stored at -80 °C. Cell number was counted again. Quantification of metabolite levels in unspent (day 1) and day 2 (conditioned media) cell culture media samples was performed.
Sample Type:Cell culture media
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