Summary of Study ST002531
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002531 |
| Study Title | Cellular consumption/release of polar metabolites by mPDAC cells cultured in different culture media conditions |
| Study Summary | We used quantitative LC-MS metabolite profiling to perform analysis of 108 metabolites that mPDAC cells consume or release while growing in RPMI or TIFM media. |
| Institute | University of Chicago |
| Last Name | Apiz Saab |
| First Name | Juan |
| Address | 929 E. 57th St. |
| japizsaab@uchicago.edu | |
| Phone | 7738346506 |
| Submit Date | 2023-03-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-13 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002637 |
| Sampleprep Summary: | We extracted metabolites from 5µL of cell culture media samples using 45µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix with labelled stable isotope internal standards. Samples in extraction mix were vortexed for 10 min at 4°C and centrifugated at 15,000x rpm for 10 min at 4°C to pellet insoluble material. 20µL of the soluble polar metabolite supernatant was moved to sample vials for analysis by LC-MS. |
