Summary of Study ST002556
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001648. The data can be accessed directly via it's Project DOI: 10.21228/M8PF0K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002556 |
| Study Title | Blood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis |
| Study Type | Case-Control Study |
| Study Summary | The objective of this study was to identify metabolites associated with metritis and use them for identification of cellular mechanisms affected during transition into lactation. Holstein cows (n = 104) had blood collected in the prepartum period (d-14 ± 6), at calving (d0), and at the day of metritis diagnosis (d7 ± 2). Cows with reddish or brownish, watery, and fetid discharge were diagnosed with metritis (n = 52). Cows with metritis were paired with herdmates without metritis (n = 52) based on DIM. The metabolome of plasma samples was evaluated using untargeted gas chromatography time-of-flight mass spectrometry. Univariate analyses included t-tests and fold change analyses. Metabolites with false discovery rate (FDR) adjusted P ≤ 0.10 on t-tests were used for partial least squares – discriminant analysis PLS-DA coupled with permutational analysis using 2,000 permutations. Metabolites with FDR adjusted P ≤ 0.10 on t-tests were also used for enriched pathway analyses and identification of cellular processes. Cows that developed metritis had affected cellular processes associated with lower amino acid metabolism in the prepartum period, greater lipolysis, cell death, and oxidative stress at calving and at metritis diagnosis, and greater leukocyte activation at calving, but lower immune cell activation at metritis diagnosis. In summary, cows that developed metritis had plasma metabolomic changes associated with greater lipolysis, oxidative stress, and a dysregulated immune response which may predispose cows to metritis development. |
| Institute | University of Florida |
| Department | College of Veterinary Medicine |
| Laboratory | Large Animal Clinical Sciences |
| Last Name | segundocasaro |
| First Name | Segundo |
| Address | 117 Deriso Hall, 2015 SW 16th Ave. |
| segundocasaro@ufl.edu | |
| Phone | 3522844016 |
| Submit Date | 2023-04-11 |
| Num Groups | 2 |
| Total Subjects | 104 |
| Num Females | 104 |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-04-28 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002650 |
| Collection Summary: | All cows had blood collected in the prepartum period (-14 DRP), calving (first 24h after calving), and at diagnosis (day of metritis diagnosis). Blood was sampled from the jugular vein using a 20-gauge x 2.54-cm needle and 10-mL evacuated tube containing lithium heparin (Vacutainer, Becton, Dickinson and Company, Franklin Lakes, NJ, USA). After collection, the blood tubes were placed on ice and transported to the laboratory within 2 hours. Once in the laboratory, the blood tubes were centrifuged at 4000 g, 4 °C, for 10 min, and the plasma was stored at -80 oC for further characterization of the plasma metabolome. The frozen plasma was submitted to the University of California’s West Coast Metabolomics Center in Davis, CA for metabolome analysis. Samples were analyzed by blinded technicians using untargeted gas chromatography with time-of-flight mass spectrometry (GC-TOF-MS) in a single batch. |
| Sample Type: | Blood (plasma) |
