Summary of Study ST002556

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001648. The data can be accessed directly via it's Project DOI: 10.21228/M8PF0K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study ID	ST002556
Study Title	Blood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis
Study Type	Case-Control Study
Study Summary	The objective of this study was to identify metabolites associated with metritis and use them for identification of cellular mechanisms affected during transition into lactation. Holstein cows (n = 104) had blood collected in the prepartum period (d-14 ± 6), at calving (d0), and at the day of metritis diagnosis (d7 ± 2). Cows with reddish or brownish, watery, and fetid discharge were diagnosed with metritis (n = 52). Cows with metritis were paired with herdmates without metritis (n = 52) based on DIM. The metabolome of plasma samples was evaluated using untargeted gas chromatography time-of-flight mass spectrometry. Univariate analyses included t-tests and fold change analyses. Metabolites with false discovery rate (FDR) adjusted P ≤ 0.10 on t-tests were used for partial least squares – discriminant analysis PLS-DA coupled with permutational analysis using 2,000 permutations. Metabolites with FDR adjusted P ≤ 0.10 on t-tests were also used for enriched pathway analyses and identification of cellular processes. Cows that developed metritis had affected cellular processes associated with lower amino acid metabolism in the prepartum period, greater lipolysis, cell death, and oxidative stress at calving and at metritis diagnosis, and greater leukocyte activation at calving, but lower immune cell activation at metritis diagnosis. In summary, cows that developed metritis had plasma metabolomic changes associated with greater lipolysis, oxidative stress, and a dysregulated immune response which may predispose cows to metritis development.
Institute	University of Florida
Department	College of Veterinary Medicine
Laboratory	Large Animal Clinical Sciences
Last Name	segundocasaro
First Name	Segundo
Address	117 Deriso Hall, 2015 SW 16th Ave.
Email	segundocasaro@ufl.edu
Phone	3522844016
Submit Date	2023-04-11
Num Groups	2
Total Subjects	104
Num Females	104
Analysis Type Detail	GC-MS
Release Date	2023-04-28
Release Version	1

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Factors:

Subject type: Mammal; Subject species: Bos taurus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Time	Parity
SA256898	9794_Calving_Con	Con	Calving	Mult
SA256899	9778_Calving_Con	Con	Calving	Mult
SA256900	9979_Calving_Con	Con	Calving	Mult
SA256901	9971_Calving_Con	Con	Calving	Mult
SA256902	9802_Calving_Con	Con	Calving	Mult
SA256903	9966_Calving_Con	Con	Calving	Mult
SA256904	9804_Calving_Con	Con	Calving	Mult
SA256905	9536_Calving_Con	Con	Calving	Mult
SA256906	9985_Calving_Con	Con	Calving	Mult
SA256907	9280_Calving_Con	Con	Calving	Mult
SA256908	9268_Calving_Con	Con	Calving	Mult
SA256909	9945_Calving_Con	Con	Calving	Mult
SA256910	10001_Calving_Con	Con	Calving	Mult
SA256911	9992_Calving_Con	Con	Calving	Mult
SA256912	9823_Calving_Con	Con	Calving	Mult
SA256913	9987_Calving_Con	Con	Calving	Mult
SA256914	9509_Calving_Con	Con	Calving	Mult
SA256915	10038_Calving_Con	Con	Calving	Mult
SA256916	9924_Calving_Con	Con	Calving	Mult
SA256917	9918_Calving_Con	Con	Calving	Mult
SA256918	9917_Calving_Con	Con	Calving	Mult
SA256919	9925_Calving_Con	Con	Calving	Mult
SA256920	9927_Calving_Con	Con	Calving	Mult
SA256921	9957_Calving_Con	Con	Calving	Mult
SA256922	9944_Calving_Con	Con	Calving	Mult
SA256923	9950_Calving_Con	Con	Calving	Mult
SA256924	9942_Calving_Con	Con	Calving	Mult
SA256925	9954_Calving_Con	Con	Calving	Mult
SA256926	9956_Calving_Con	Con	Calving	Mult
SA256927	9875_Calving_Con	Con	Calving	Mult
SA256928	9872_Calving_Con	Con	Calving	Mult
SA256929	9904_Calving_Con	Con	Calving	Mult
SA256930	9876_Calving_Con	Con	Calving	Mult
SA256931	9907_Calving_Con	Con	Calving	Mult
SA256932	10303_Calving_Con	Con	Calving	Prim
SA256933	10264_Calving_Con	Con	Calving	Prim
SA256934	10254_Calving_Con	Con	Calving	Prim
SA256935	10212_Calving_Con	Con	Calving	Prim
SA256936	10246_Calving_Con	Con	Calving	Prim
SA256937	10262_Calving_Con	Con	Calving	Prim
SA256938	10268_Calving_Con	Con	Calving	Prim
SA256939	10274_Calving_Con	Con	Calving	Prim
SA256940	10311_Calving_Con	Con	Calving	Prim
SA256941	10261_Calving_Con	Con	Calving	Prim
SA256942	10229_Calving_Con	Con	Calving	Prim
SA256943	10290_Calving_Con	Con	Calving	Prim
SA256944	10245_Calving_Con	Con	Calving	Prim
SA256945	10194_Calving_Con	Con	Calving	Prim
SA256946	10171_Calving_Con	Con	Calving	Prim
SA256947	10165_Calving_Con	Con	Calving	Prim
SA256948	10199_Calving_Con	Con	Calving	Prim
SA256949	10189_Calving_Con	Con	Calving	Prim
SA256950	9985_Diagnosis_Con	Con	Diagnosis	Mult
SA256951	9904_Diagnosis_Con	Con	Diagnosis	Mult
SA256952	9907_Diagnosis_Con	Con	Diagnosis	Mult
SA256953	9918_Diagnosis_Con	Con	Diagnosis	Mult
SA256954	9954_Diagnosis_Con	Con	Diagnosis	Mult
SA256955	9917_Diagnosis_Con	Con	Diagnosis	Mult
SA256956	9872_Diagnosis_Con	Con	Diagnosis	Mult
SA256957	9876_Diagnosis_Con	Con	Diagnosis	Mult
SA256958	9804_Diagnosis_Con	Con	Diagnosis	Mult
SA256959	9794_Diagnosis_Con	Con	Diagnosis	Mult
SA256960	9778_Diagnosis_Con	Con	Diagnosis	Mult
SA256961	9536_Diagnosis_Con	Con	Diagnosis	Mult
SA256962	9802_Diagnosis_Con	Con	Diagnosis	Mult
SA256963	9268_Diagnosis_Con	Con	Diagnosis	Mult
SA256964	9509_Diagnosis_Con	Con	Diagnosis	Mult
SA256965	9823_Diagnosis_Con	Con	Diagnosis	Mult
SA256966	9280_Diagnosis_Con	Con	Diagnosis	Mult
SA256967	9875_Diagnosis_Con	Con	Diagnosis	Mult
SA256968	9925_Diagnosis_Con	Con	Diagnosis	Mult
SA256969	9979_Diagnosis_Con	Con	Diagnosis	Mult
SA256970	9971_Diagnosis_Con	Con	Diagnosis	Mult
SA256971	9987_Diagnosis_Con	Con	Diagnosis	Mult
SA256972	10038_Diagnosis_Con	Con	Diagnosis	Mult
SA256973	9992_Diagnosis_Con	Con	Diagnosis	Mult
SA256974	9924_Diagnosis_Con	Con	Diagnosis	Mult
SA256975	10001_Diagnosis_Con	Con	Diagnosis	Mult
SA256976	9966_Diagnosis_Con	Con	Diagnosis	Mult
SA256977	9944_Diagnosis_Con	Con	Diagnosis	Mult
SA256978	9942_Diagnosis_Con	Con	Diagnosis	Mult
SA256979	9927_Diagnosis_Con	Con	Diagnosis	Mult
SA256980	9957_Diagnosis_Con	Con	Diagnosis	Mult
SA256981	9945_Diagnosis_Con	Con	Diagnosis	Mult
SA256982	9956_Diagnosis_Con	Con	Diagnosis	Mult
SA256983	9950_Diagnosis_Con	Con	Diagnosis	Mult
SA256984	10254_Diagnosis_Con	Con	Diagnosis	Prim
SA256985	10194_Diagnosis_Con	Con	Diagnosis	Prim
SA256986	10171_Diagnosis_Con	Con	Diagnosis	Prim
SA256987	10189_Diagnosis_Con	Con	Diagnosis	Prim
SA256988	10261_Diagnosis_Con	Con	Diagnosis	Prim
SA256989	10165_Diagnosis_Con	Con	Diagnosis	Prim
SA256990	10199_Diagnosis_Con	Con	Diagnosis	Prim
SA256991	10212_Diagnosis_Con	Con	Diagnosis	Prim
SA256992	10245_Diagnosis_Con	Con	Diagnosis	Prim
SA256993	10262_Diagnosis_Con	Con	Diagnosis	Prim
SA256994	10229_Diagnosis_Con	Con	Diagnosis	Prim
SA256995	10274_Diagnosis_Con	Con	Diagnosis	Prim
SA256996	10303_Diagnosis_Con	Con	Diagnosis	Prim
SA256997	10311_Diagnosis_Con	Con	Diagnosis	Prim