Summary of Study ST002752

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002752
Study TitleBiomolecular condensates create phospholipid-enriched microenvironments (Part 7 - reversed phase experiment set 2)
Study TypeMetabolomes of in vitro synthesized condensates
Study SummaryProteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. In this project we used mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1. In this sub-study, we examined the metabolomes of the mouse liver samples that were used to conduct the condensate metabolome experiment described above.
Cornell University
DepartmentDepartment of Pharmacology
LaboratoryDr. Samie Jaffrey
Last NameDumelie
First NameJason
Address1300 York Ave, LC-524, New York City, NY
Submit Date2023-06-22
Raw Data AvailableYes
Raw Data File Type(s)xml
Analysis Type DetailLC-MS
Release Date2023-07-10
Release Version1
Jason Dumelie Jason Dumelie application/zip

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Collection ID:CO002852
Collection Summary:Mouse metabolites were collected from the liver of female mice using methanol extraction. After euthanizing a mouse, the liver was immediately frozen in liquid nitrogen. We then used cold 80% methanol to extract metabolites. First, 1 ml of 80% methanol was added to the liver and incubated for 10 min at -20oC. Glass beads were added to the liver and then the liver was lysed by bead-beating for 45 s using a Tissuelyser cell disrupter (Qiagen). The lysate was incubated for 10 min at -20oC and centrifuged (13200 rpm, 5 min) to separate metabolites from macromolecules. The supernatant was collected and 200 µl of 80% methanol was added to the pellet. The incubation, shaking and centrifugation steps were repeated twice to extract more metabolites from the pellet. The three supernatants were combined and centrifuged (14000 rpm, 10 min) to separate any remaining macromolecules from the metabolites. The combined supernatants were dried using a SpeedVac Concentrator (Savant, SPD131DDA) at 25oC and the dried metabolite samples were stored at -80oC.
Sample Type:Liver
Collection Method:80% methanol
Storage Conditions:-80℃