Summary of Study ST002804
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001751. The data can be accessed directly via it's Project DOI: 10.21228/M8CH9W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002804 |
| Study Title | Metabolic Adaptations of Microbacterium sediminis YLB-01 for Survival in the Challenging High-Pressure of the Deep Sea |
| Study Summary | By using NMR-based metabolomic analysis, we investigated the metabolic adaptations of Microbacterium sediminis YLB-01 in response to high-pressure conditions. We recorded the 600 MHz 1D 1H-NMR spectra on aqueous extracts from YLB-01 cells, and then assigned resonances of 31 metabolites. The distinct metabolic separation between the HPLT and NPLT groups highlighted the significant effect of high-pressure treatment on the metabolism of YLB-01 cells. |
| Institute | Xiamen University |
| Last Name | Qiu |
| First Name | Xu |
| Address | No. 422, Siming South Road, Xiamen, Fujian, China. |
| qiuxu@stu.xmu.edu.cn | |
| Phone | 13161342734 |
| Submit Date | 2023-06-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-08-22 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002904 |
| Collection Summary: | In this study, a single colony of the YLB-01 strain was inoculated from an agar plate into individual test tubes containing 5 mL of TSB medium. The test tubes were then incubated in a shaker at 28°C for 12 h. Subsequently, the seed cultures were transferred to 150-mL Erlenmeyer flasks at a ratio of 1:20, with each flask containing 100 mL of TSB medium. To ensure an adequate cell count, YLB-01 cultures were initially cultivated under optimal conditions (28°C, 0.1 MPa) for 24 h. The optical density at 600 nm (OD600 nm) of the cell cultures was measured using an automatic growth curve analyzer (FP-1100-C, Growth Curves Ab Ltd., Finland). Once the cultures reached the stationary phase of growth, the cells were transferred into a 100-mL sterile saline bag and placed in a high-pressure culture kettle. This kettle, obtained from Nantong Feiyu Company, China, was specifically designed to simulate the high-pressure conditions of the deep sea (Zhang et al. 2015). The cells were then exposed to either 30 MPa (the HPLT group, N=8) or 0.1 MPa (the NPLT group, N=7) at 4°C for 7 days. Hydrostatic pressure was generated by injecting pure water into the vessel. |
| Sample Type: | Bacterial cells |
| Storage Conditions: | Described in summary |
