Summary of Study ST002804

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001751. The data can be accessed directly via it's Project DOI: 10.21228/M8CH9W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002804
Study TitleMetabolic Adaptations of Microbacterium sediminis YLB-01 for Survival in the Challenging High-Pressure of the Deep Sea
Study SummaryBy using NMR-based metabolomic analysis, we investigated the metabolic adaptations of Microbacterium sediminis YLB-01 in response to high-pressure conditions. We recorded the 600 MHz 1D 1H-NMR spectra on aqueous extracts from YLB-01 cells, and then assigned resonances of 31 metabolites. The distinct metabolic separation between the HPLT and NPLT groups highlighted the significant effect of high-pressure treatment on the metabolism of YLB-01 cells.
Institute
Xiamen University
Last NameQiu
First NameXu
AddressNo. 422, Siming South Road, Xiamen, Fujian, China.
Emailqiuxu@stu.xmu.edu.cn
Phone13161342734
Submit Date2023-06-18
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2023-08-22
Release Version1
Xu Qiu Xu Qiu
https://dx.doi.org/10.21228/M8CH9W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002920
Treatment Summary:To ensure an adequate cell count, YLB-01 cultures were initially cultivated under optimal conditions (28°C, 0.1 MPa) for 24 h. The optical density at 600 nm (OD600 nm) of the cell cultures was measured using an automatic growth curve analyzer (FP-1100-C, Growth Curves Ab Ltd., Finland). Once the cultures reached the stationary phase of growth, the cells were transferred into a 100-mL sterile saline bag and placed in a high-pressure culture kettle. The cells were then exposed to either 30 MPa (the HPLT group, N=8) or 0.1 MPa (the NPLT group, N=7) at 4°C for 7 days.
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