Summary of Study ST002914
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001812. The data can be accessed directly via it's Project DOI: 10.21228/M8GX42 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002914 |
| Study Title | Untangling the Dynamics of Lysine Acetylation and Phosphorylation in Adipogenesis in the Established Human and Mouse Adipocyte Cell Lines SGBS and 3T3L1 |
| Study Summary | Obesity is one of the most pressing global public health challenges of our time with yet increasing prevalence. Characterized by enlarged and dysfunctional adipose tissue, it is often associated with the development of metabolic or cardiovascular comorbidities. A sound understanding of the processes underlying adipogenesis, the differentiation of a preadipocyte into a mature and lipid laden adipocyte, provide the basic knowledge for future research on the causes and consequences of obesity. The tricarboxylic acid cycle represents the central metabolic hub, that provides energy equivalents, metabolic building blocks and critical intermediates such as acetyl-CoA as precursor for protein acetylation and de novo lipogenesis. Stable cell lines are an integral part of research into the development and physiology of adipocytes in health and disease and various models have been used to date. In this study we demonstrated the vivid temporal dynamics of central carbon metabolites during the differentiation of SGBS and 3T3-L1 adipocytes. Detected metabolite levels showed distinct temporal profiles, partly with cell-line specificities. |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Molecular Systems Biology |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstraße 15, Leipzipg, Saxony, 03418, Germany |
| beatrice.engelmann@ufz.de | |
| Phone | 00493412351099 |
| Submit Date | 2023-09-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-10-27 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003020 |
| Collection Summary: | Human SGBS cells were provided by the laboratory of Prof. Dr. Wabitsch at the University Clinic Ulm. Cells were differentiated according to the standard protocol described previously (Wabitsch et al., 2001). 3T3-L1 mouse embryonic fibroblasts were purchased from the American Type Culture Collection (ATCC, CL-173, USA). |
| Sample Type: | Adipose tissue |
| Storage Conditions: | -80℃ |
