Summary of Study ST002914

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001812. The data can be accessed directly via it's Project DOI: 10.21228/M8GX42 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002914
Study Title	Untangling the Dynamics of Lysine Acetylation and Phosphorylation in Adipogenesis in the Established Human and Mouse Adipocyte Cell Lines SGBS and 3T3L1
Study Summary	Obesity is one of the most pressing global public health challenges of our time with yet increasing prevalence. Characterized by enlarged and dysfunctional adipose tissue, it is often associated with the development of metabolic or cardiovascular comorbidities. A sound understanding of the processes underlying adipogenesis, the differentiation of a preadipocyte into a mature and lipid laden adipocyte, provide the basic knowledge for future research on the causes and consequences of obesity. The tricarboxylic acid cycle represents the central metabolic hub, that provides energy equivalents, metabolic building blocks and critical intermediates such as acetyl-CoA as precursor for protein acetylation and de novo lipogenesis. Stable cell lines are an integral part of research into the development and physiology of adipocytes in health and disease and various models have been used to date. In this study we demonstrated the vivid temporal dynamics of central carbon metabolites during the differentiation of SGBS and 3T3-L1 adipocytes. Detected metabolite levels showed distinct temporal profiles, partly with cell-line specificities.
Institute	Helmholtz Centre for Environmental Research
Department	Molecular Systems Biology
Last Name	Engelmann
First Name	Beatrice
Address	Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
Email	beatrice.engelmann@ufz.de
Phone	00493412351099
Submit Date	2023-09-26
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2023-10-27
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003033
Sampleprep Summary:	Intracellular metabolites were extracted using a 1:1:1 methanol:water:chloroform extraction protocol. Briefly, culture media was removed and cells were rinsed with icecold 0.9 % sodium chloride solution. The rinsing solution was removed and the cells metabolism was quenched by adding equal volumes of methanol, followed by icecold deionized water. Cells were scraped off the culture plate and combined chloroform. Samples were vortexed and kept on a shaker at 4°C for 20 min at 1400 rpm. Cell debris was removed by centrifugation. A fixed volume of the polar upper phase was transferred to new tubes and dried to completeness.

Sampleprep ID:

SP003033

Sampleprep Summary:

Intracellular metabolites were extracted using a 1:1:1 methanol:water:chloroform extraction protocol. Briefly, culture media was removed and cells were rinsed with icecold 0.9 % sodium chloride solution. The rinsing solution was removed and the cells metabolism was quenched by adding equal volumes of methanol, followed by icecold deionized water. Cells were scraped off the culture plate and combined chloroform. Samples were vortexed and kept on a shaker at 4°C for 20 min at 1400 rpm. Cell debris was removed by centrifugation. A fixed volume of the polar upper phase was transferred to new tubes and dried to completeness.