Summary of Study ST003066
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001912. The data can be accessed directly via it's Project DOI: 10.21228/M8KB0M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003066 |
| Study Title | Heritability of RBC metabolites: baseline correlation of metabolites and markers of RBC health and stability |
| Study Summary | The goal of this study was to examine the genetic heritability of metabolites and to identify metabolites, present immediately after blood draw, that were highly correlated with red blood cells (RBCs) ex vivo survival. Extreme longevity in humans is known to be a heritable trait. In a well-established twin erythrocyte metabolomics and proteomics database, we identified the longevity factor spermidine and a cluster of correlated molecules with high heritability estimates. Erythrocyte spermidine is 82% heritable and significantly correlated with 59 metabolites and 22 proteins. Thirty-eight metabolites and 19 proteins were >20% heritable, with a mean heritability of 61% for metabolites and 49% for proteins. Correlated metabolites are concentrated in energy metabolism, redox homeostasis, and autophagy pathways. Erythrocyte mean cell volume (MCV)−an established heritable trait−was consistently negatively correlated with the top 25 biomolecules most strongly correlated with spermidine, indicating that smaller MCVs are associated with higher concentrations of spermidine and correlated molecules. |
| Institute | University of Iowa |
| Last Name | Raife |
| First Name | Thomas |
| Address | 1685 Highland Ave. Madison WI 53705 |
| traife@wisc.edu | |
| Phone | (608)-265-8492 |
| Submit Date | 2024-02-02 |
| Analysis Type Detail | Other |
| Release Date | 2024-02-28 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003174 |
| Collection Summary: | This study utilized a previously reported twin study database comprising metabolomics and proteomics of erythrocytes in adult twins. The study was approved by the Human Subjects office of The University of Iowa Carver College of Medicine. Written informed consent was obtained from all participating subjects. Subjects qualified for participation by meeting criteria for autologous blood donation according to standard operating procedures of The University of Iowa DeGowin Blood Center. Five dizygotic (DZ) and 13 monozygotic (MZ) twin pairs participated in this study, but twin pairs were not required to donate samples concurrently. Standard health history and demographic information was obtained at the time of enrollment and informed consent. Whole venous blood (EDTA, Vacutainer® purple top blood collection tube, 8 ml) collected from participants prior to blood donation was centrifuged at 500 × g for 5 min, followed by removal of the plasma and buffy coat. RBCs were washed twice with cold isotonic saline solution. After washing, a 30 μl aliquot of the packed red blood cells (pRBCs) was removed for complete blood count (CBC) analysis (Sysmex XE-2100™ Automated Hematology System, Sysmex Corp). A 100 μl aliquot of pRBCs was lysed with 900 μl of nanopure water. Samples were thoroughly mixed and stored at −80 °C prior to proteomic and metabolomic analyses. |
| Sample Type: | Erythrocytes |
