Summary of Study ST000495

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000372. The data can be accessed directly via it's Project DOI: 10.21228/M8ZP5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000495
Study TitleMetabolomic profiles along the gastrointestinal tract of the healthy dog
Study SummaryIntroduction: The fecal microbiome is relevant to the health and disease of many species. The importance of the fecal metabolome has more recently been appreciated, but our knowledge of the microbiome and metabolome at other sites along the gastrointestinal tract remains deficient. Objective: To analyze the gastrointestinal microbiome and metabolome of healthy domestic dogs at four anatomical sites. Methods: Samples of the duodenal, ileal, colonic, and rectal contents were collected from six adult dogs after humane euthanasia for an unrelated study. The microbiota were characterized using Illumina sequencing of 16S rRNA genes. The metabolome was characterized by mass spectrometry-based methods. Results: Prevalent phyla throughout the samples were Proteobacteria, Firmicutes, Fusobacteria, and Bacteroidetes, consistent with previous findings in dogs and other species. A total of 530 unique metabolites were detected; 199 of these were identified as previously named compounds, but 141 of them had at least one significantly different site-pair comparison. Noteworthy examples include amino acids, which decreased from the small to large intestine; pyruvate, which was at peak concentrations in the ileum; and several phenol-containing carboxylic acid compounds that increased in the large intestine. Conclusion: The microbiome and metabolome vary significantly at different sites along the canine gastrointestinal tract.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2016-10-17
Num Groups4
Total Subjects24
Num Males4
Num Females2
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2016-12-22
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M8ZP5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Animal; Subject species: Canis lupus familiaris (Factor headings shown in green)

mb_sample_id local_sample_id Organ Sex
SA025625141217actsa05_1colon Female
SA025626141217actsa19_1colon Female
SA025627141217actsa23_1colon Male
SA025628141217actsa22_1colon Male
SA025629141217actsa10_1colon Male
SA025630141217actsa04_1colon Male
SA025631141217actsa17_1duod Female
SA025632141217actsa13_1duod Female
SA025633141217actsa24_1duod Male
SA025634141217actsa21_1duod Male
SA025635141217actsa20_1duod Male
SA025636141217actsa09_1duod Male
SA025637141217actsa18_1ileum Female
SA025638141217actsa07_1ileum Female
SA025639141217actsa02_1ileum Male
SA025640141217actsa01_1ileum Male
SA025641141217actsa15_1ileum Male
SA025642141217actsa14_1ileum Male
SA025643141217actsa06_1rectum Female
SA025644141217actsa16_1rectum Female
SA025645141217actsa03_1rectum Male
SA025646141217actsa12_1rectum Male
SA025647141217actsa11_1rectum Male
SA025648141217actsa08_1rectum Male
Showing results 1 to 24 of 24
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