Summary of Study ST000495

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000372. The data can be accessed directly via it's Project DOI: 10.21228/M8ZP5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000495
Study TitleMetabolomic profiles along the gastrointestinal tract of the healthy dog
Study SummaryIntroduction: The fecal microbiome is relevant to the health and disease of many species. The importance of the fecal metabolome has more recently been appreciated, but our knowledge of the microbiome and metabolome at other sites along the gastrointestinal tract remains deficient. Objective: To analyze the gastrointestinal microbiome and metabolome of healthy domestic dogs at four anatomical sites. Methods: Samples of the duodenal, ileal, colonic, and rectal contents were collected from six adult dogs after humane euthanasia for an unrelated study. The microbiota were characterized using Illumina sequencing of 16S rRNA genes. The metabolome was characterized by mass spectrometry-based methods. Results: Prevalent phyla throughout the samples were Proteobacteria, Firmicutes, Fusobacteria, and Bacteroidetes, consistent with previous findings in dogs and other species. A total of 530 unique metabolites were detected; 199 of these were identified as previously named compounds, but 141 of them had at least one significantly different site-pair comparison. Noteworthy examples include amino acids, which decreased from the small to large intestine; pyruvate, which was at peak concentrations in the ileum; and several phenol-containing carboxylic acid compounds that increased in the large intestine. Conclusion: The microbiome and metabolome vary significantly at different sites along the canine gastrointestinal tract.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2016-10-17
Num Groups4
Total Subjects24
Num Males4
Num Females2
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2016-12-22
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M8ZP5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU000516
Subject Type:Animal
Subject Species:Canis lupus familiaris
Taxonomy ID:9615
Genotype Strain:Hound-type
Age Or Age Range:Adult
Weight Or Weight Range:35-60 lbs
Gender:Male/Female
Animal Inclusion Criteria:no evidence of gastrointestinal illness or other disease
Species Group:Mammal
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