Summary of Study ST001920

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001211. The data can be accessed directly via it's Project DOI: 10.21228/M85Q6Z This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001920
Study TitleMetabolic and lipidomic characterization of radioresistant MDA-MB-231 human breast cancer cells to investigate potential therapeutic targets
Study SummaryTo provide preliminary insights into metabolic and lipidomic characteristics in radioresistant triple-negative breast cancer (TNBC) cells and suggest potential therapeutic targets, we performed a comprehensive metabolic and lipidomic profiling of radioresistant MDA-MB-231 (MDA-MB-231/RR) TNBC cells and their parental cells using gas chromatography-mass spectrometry and nano electrospray ionization-mass spectrometry, followed by multivariate statistical analysis. Buthionine sulfoximine (BSO) and radiation were co-treated to radioresistant TNBC cells. The level of glutathione (GSH) was significantly increased, and the levels of GSH synthesis-related metabolites, such as cysteine, glycine, and glutamine were also increased in MDA-MB-231/RR cells. In contrast, the level of lactic acid was significantly reduced. In addition, reactive oxygen species (ROS) level was decreased in MDA-MB-231/RR cells. In the lipidomic profiles of MDA-MB-231/RR cells, the levels of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were significantly increased, whereas those of most of the phosphatidylinositol species were significantly decreased. BSO sensitized MDA-MB-231/RR cells to radiotherapy, which resulted in decreased GSH level and increased ROS level and apoptosis. Radioresistant TNBC cells showed distinct metabolic and lipidomic characteristics compared to their parental cells. We suggested activated GSH, PC, and PE biosynthesis pathways as potential targets for treating radioresistant TNBC cells. Particularly, enhanced radiosensitivity was achieved by inhibition of GSH biosynthesis in MDA-MB-231/RR cells.
Institute
ChungAng University
DepartmentCollege of Pharmacy
LaboratoryNatural product biotechnology and Metabolomics
Last NameLee
First NameHwanhui
AddressCollege of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea
Emailhwanhui56@gmail.com
Phone+8228205605
Submit Date2021-09-22
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo), d
Analysis Type DetailGC-MS/MS(Dir. inf.)
Release Date2021-10-02
Release Version1
Hwanhui Lee Hwanhui Lee
https://dx.doi.org/10.21228/M85Q6Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factors
SA177871MDAMB231_4_2Factors;Control
SA177872MDAMB231_4_1Factors;Control
SA177873MDAMB231_4_3Factors;Control
SA177874MDAMB231_5_3Factors;Control
SA177875MDAMB231_1_1Factors;Control
SA177876MDAMB231_3_3Factors;Control
SA177877MDAMB231_5_2Factors;Control
SA177878MDAMB231_5_1Factors;Control
SA177879MDAMB231_1_3Factors;Control
SA177880MDAMB231_3_2Factors;Control
SA177881MDAMB231_1_2Factors;Control
SA177882MDAMB231_2_1Factors;Control
SA177883MDAMB231_2_2Factors;Control
SA177884MDAMB231_3_1Factors;Control
SA177885MDAMB231_2_3Factors;Control
SA177886QC_2Factors;Quality control
SA177887QC_1Factors;Quality control
SA177888QC_3Factors;Quality control
SA177889QC_5Factors;Quality control
SA177890QC_4Factors;Quality control
SA177891QC_6Factors;Quality control
SA177892MDAMB231RR_5_1Factors;radioresistant cells
SA177893MDAMB231RR_5_2Factors;radioresistant cells
SA177894MDAMB231RR_4_3Factors;radioresistant cells
SA177895MDAMB231RR_5_3Factors;radioresistant cells
SA177896MDAMB231RR_2_1Factors;radioresistant cells
SA177897MDAMB231RR_2_2Factors;radioresistant cells
SA177898MDAMB231RR_1_3Factors;radioresistant cells
SA177899MDAMB231RR_1_2Factors;radioresistant cells
SA177900MDAMB231RR_1_1Factors;radioresistant cells
SA177901MDAMB231RR_2_3Factors;radioresistant cells
SA177902MDAMB231RR_3_1Factors;radioresistant cells
SA177903MDAMB231RR_4_1Factors;radioresistant cells
SA177904MDAMB231RR_3_3Factors;radioresistant cells
SA177905MDAMB231RR_3_2Factors;radioresistant cells
SA177906MDAMB231RR_4_2Factors;radioresistant cells
Showing results 1 to 36 of 36
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