Summary of Study ST002106

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001335. The data can be accessed directly via it's Project DOI: 10.21228/M85416 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002106
Study TitleGenetic and chemical validation of Plasmodium falciparum aminopeptidase PfA-M17 as a drug target in the hemoglobin digestion pathway (Part 1)
Study SummaryPlasmodium falciparum, the causative agent of malaria, continues to remain a global health threat since these parasites are now resistant to all anti-malaria drugs used throughout the world. Accordingly, drugs with novel modes of action are desperately required to combat malaria. P. falciparum parasites infect human red blood cells where they digest the hosts main protein constituent, hemoglobin. Leucine aminopeptidase PfA-M17 is one of several aminopeptidases that have been implicated in the last step of this digestive pathway. Here we utilize both reverse genetics and a compound specifically designed to inhibit the activity of PfA-M17 to show that PfA-M17 is essential for P. falciparum survival as it provides parasites with free amino acids for growth, many of which are highly likely to originate from hemoglobin. We further show that our inhibitor is on-target for PfA-M17 and has the ability to kill parasites at nanomolar concentrations. Thus, in contrast to other hemoglobin-degrading proteases that have overlapping redundant functions, we validate PfA-M17 as a potential novel drug target.
Monash University
Last NameSiddiqui
First NameGhizal
Address381 Royal Parade, Parkville, Melbourne, Victoria, 3052, Australia
Submit Date2022-03-16
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-04-04
Release Version1
Ghizal Siddiqui Ghizal Siddiqui application/zip

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Subject type: Cultured cells; Subject species: Plasmodium falciparum (Factor headings shown in green)

mb_sample_id local_sample_id cell_type treatment
SA202321P_3D7_MIPS2571_1iRBC 3D7 + Compound 3-1
SA202322P_3D7_MIPS2571_2iRBC 3D7 + Compound 3-2
SA202323P_3D7_MIPS2571_3iRBC 3D7 + Compound 3-3
SA202324P_3D7_gluc_1iRBC 3D7 + Glucosamine -1
SA202325P_3D7_gluc_2iRBC 3D7 + Glucosamine -2
SA202326P_3D7_gluc_3iRBC 3D7 + Glucosamine -3
SA202327P_3D7_1iRBC DMSO -1
SA202328P_3D7_2iRBC DMSO -2
SA202329P_3D7_3iRBC DMSO -3
SA202333P_2E7_1iRBC PfAM17-HAglmS-1
SA202334P_2E7_2iRBC PfAM17-HAglmS-2
SA202335P_2E7_3iRBC PfAM17-HAglmS-3
SA202330P_2E7_gluc_1iRBC PfAM17-HAglmS +Glucosamine -1
SA202331P_2E7_gluc_2iRBC PfAM17-HAglmS +Glucosamine -2
SA202332P_2E7_gluc_3iRBC PfAM17-HAglmS +Glucosamine -3
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