Summary of Study ST002199

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001402. The data can be accessed directly via it's Project DOI: 10.21228/M8H42P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002199
Study TitleFOXA2 controls the anti-oxidant response in FH-deficient cells independent of NRF2
Study SummaryHereditary Leiomyomatosis and renal cell cancer is caused by fumarate hydratase loss of heterozygosity and subsequence accumulation of fumarate. Fumarate is known to activate the anti-oxidant response and is key for cellular survival. Fumarate succinates KEAP1 which releases NRF2 to activate the antioxidant response. The role of fumarate on the global regulatory chromatin landscape is less understood. Here, by integrating chromatin accessibility and histone ChIP-seq profiles, we identify complex transcription factor networks involved in the highly remodelled chromatin landscape of FH-deficient cells. We implicate FOXA2 in the maintenance of FH-deficient cells by regulating anti-oxidant response genes and subsequent metabolic output, independent of NRF2. These results identify new redox and amino acid metabolism regulators and provide new avenues for therapeutic intervention.
Institute
CECAD Research Center
Last NameYang
First NameMing
AddressJoseph-Stelzmann-Straße 26, Köln, Koeln, 50931, Germany
Emailming.yang@uni-koeln.de
Phone4922147884306
Submit Date2022-06-01
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-07-14
Release Version1
Ming Yang Ming Yang
https://dx.doi.org/10.21228/M8H42P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA210708CR02_056Fh1-/-CL19 siFoxa2
SA210709CR02_057Fh1-/-CL19 siFoxa2
SA210710CR02_058Fh1-/-CL19 siFoxa2
SA210711CR02_059Fh1-/-CL19 siFoxa2
SA210712CR02_060Fh1-/-CL19 siFoxa2
SA210713CR02_052Fh1-/-CL19 siNT
SA210714CR02_051Fh1-/-CL19 siNT
SA210715CR02_053Fh1-/-CL19 siNT
SA210716CR02_055Fh1-/-CL19 siNT
SA210717CR02_054Fh1-/-CL19 siNT
SA210718CR02_048Fh1-/-CL1 siFoxa2
SA210719CR02_049Fh1-/-CL1 siFoxa2
SA210720CR02_050Fh1-/-CL1 siFoxa2
SA210721CR02_047Fh1-/-CL1 siFoxa2
SA210722CR02_046Fh1-/-CL1 siFoxa2
SA210723CR02_041Fh1-/-CL1 siNT
SA210724CR02_042Fh1-/-CL1 siNT
SA210725CR02_043Fh1-/-CL1 siNT
SA210726CR02_044Fh1-/-CL1 siNT
SA210727CR02_045Fh1-/-CL1 siNT
SA210728CR02_037Fh1fl/fl siFoxa2
SA210729CR02_036Fh1fl/fl siFoxa2
SA210730CR02_038Fh1fl/fl siFoxa2
SA210731CR02_039Fh1fl/fl siFoxa2
SA210732CR02_040Fh1fl/fl siFoxa2
SA210733CR02_032Fh1fl/fl siNT
SA210734CR02_033Fh1fl/fl siNT
SA210735CR02_035Fh1fl/fl siNT
SA210736CR02_031Fh1fl/fl siNT
SA210737CR02_034Fh1fl/fl siNT
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