Summary of Study ST002356

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001513. The data can be accessed directly via it's Project DOI: 10.21228/M8471X This work is supported by NIH grant, U2C- DK119886.

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Study IDST002356
Study TitleIsolated murine skeletal muscles utilize pyruvate over glucose for oxidation-Part 1
Study TypeStudy of the different substrate by isolated skeletal muscle at room temperature via C-13 isotopomer analysis
Study SummaryPreclinical studies of muscle contractile function often employ ex vivo preparations of the soleus and/or extensor digitorum longus (EDL) muscles which are relatively easy to prepare and represent slow and fast fiber properties, respectively. Therefore, the current study sought to examine the utility of this preparation for understanding the metabolic fuel utilization in isolated resting mouse muscles at room temperature. 13C-labeling in both muscle types was performed using three fuels: glucose, pyruvate, and acetate, followed by NMR-based metabolomics analyses. Incubating 13C-labeled substrates in the isolated skeletal muscles makes it possible to examine TCA cycle flux and substrate selection by these muscles.
Institute
University of Florida
DepartmentApplied Physiology and Kinesiology
LaboratoryRm 42 and Rm 43
Last NameKhattri
First NameRam
Address1864 Stadium RD, Gainesville, FL, 32611, USA
Emailrbk11@ufl.edu
Phone3307856045
Submit Date2022-10-31
Num Groups4
Total Subjects18
Study CommentsSoutheastern Center for Integrated Metabolomics (SECIM) (ERB), NIH AR U54 AR052646 (Physiological Assessment Core, ERB), and Wellstone Muscular Dystrophy Cooperative Research Center Grant (NIAMS: U54AR052646/P50 AR052646). The AMRIS Facility is supported by the National Science Foundation Cooperative Agreement No. DMR-1644779 and the State of Florida.
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2023-05-01
Release Version1
Ram Khattri Ram Khattri
https://dx.doi.org/10.21228/M8471X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA236886Glucose_EDL_1D_DecON&OFF-1Glucose_EDL
SA236887Glucose_EDL_1D_DecON&OFF-4Glucose_EDL
SA236888Glucose_EDL_1D_DecON&OFF-3Glucose_EDL
SA236889Glucose_EDL_1D_DecON&OFF-2Glucose_EDL
SA236890Glucose_Soleus_1D_DecON&OFF-3Glucose_Soleus
SA236891Glucose_Soleus_1D_DecON&OFF-4Glucose_Soleus
SA236892Glucose_Soleus_1D_DecON&OFF-1Glucose_Soleus
SA236893Glucose_Soleus_1D_DecON&OFF-2Glucose_Soleus
SA236894Pyruvate_EDL_1D_DecON&OFF-3Pyruvate_EDL
SA236895Pyruvate_EDL_1D_DecON&OFF-2Pyruvate_EDL
SA236896Pyruvate_EDL_1D_DecON&OFF-1Pyruvate_EDL
SA236897Pyruvate_Soleus_1D_DecON&OFF-3Pyruvate_Soleus
SA236898Pyruvate_Soleus_1D_DecON&OFF-2Pyruvate_Soleus
SA236899Pyruvate_Soleus_1D_DecON&OFF-1Pyruvate_Soleus
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