Summary of Study ST002356
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001513. The data can be accessed directly via it's Project DOI: 10.21228/M8471X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002356 |
| Study Title | Isolated murine skeletal muscles utilize pyruvate over glucose for oxidation-Part 1 |
| Study Type | Study of the different substrate by isolated skeletal muscle at room temperature via C-13 isotopomer analysis |
| Study Summary | Preclinical studies of muscle contractile function often employ ex vivo preparations of the soleus and/or extensor digitorum longus (EDL) muscles which are relatively easy to prepare and represent slow and fast fiber properties, respectively. Therefore, the current study sought to examine the utility of this preparation for understanding the metabolic fuel utilization in isolated resting mouse muscles at room temperature. 13C-labeling in both muscle types was performed using three fuels: glucose, pyruvate, and acetate, followed by NMR-based metabolomics analyses. Incubating 13C-labeled substrates in the isolated skeletal muscles makes it possible to examine TCA cycle flux and substrate selection by these muscles. |
| Institute | University of Florida |
| Department | Applied Physiology and Kinesiology |
| Laboratory | Rm 42 and Rm 43 |
| Last Name | Khattri |
| First Name | Ram |
| Address | 1864 Stadium RD, Gainesville, FL, 32611, USA |
| rbk11@ufl.edu | |
| Phone | 3307856045 |
| Submit Date | 2022-10-31 |
| Num Groups | 4 |
| Total Subjects | 18 |
| Study Comments | Southeastern Center for Integrated Metabolomics (SECIM) (ERB), NIH AR U54 AR052646 (Physiological Assessment Core, ERB), and Wellstone Muscular Dystrophy Cooperative Research Center Grant (NIAMS: U54AR052646/P50 AR052646). The AMRIS Facility is supported by the National Science Foundation Cooperative Agreement No. DMR-1644779 and the State of Florida. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-05-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000255 |
| Analysis ID: | AN003847 |
| Instrument Name: | Bruker 600 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5mM DSS |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5 mm CPTXI 1H/D-13C/15N Z-GRD Z44866/0026 |
| NMR Solvent: | Phosphate buffer (pH 7.2) + 2 mM EDTA + 0.5 mM DSS + 0.2% of sodium azide in deuterated environment |
| NMR Tube Size: | 1.5 mm NMR tube |
| Shimming Method: | Topshim |
| Pulse Sequence: | zgig1d |
| Water Suppression: | none |
| Pulse Width: | 90-degree |
| Receiver Gain: | 25.4 |
| Offset Frequency: | 4.77 ppm |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.2 oK |
| Number Of Scans: | 32 |
| Dummy Scans: | 8 |
| Acquisition Time: | 1.15s |
| Relaxation Delay: | 2.85 s |
| Spectral Width: | 7142.9 Hz |
| Num Data Points Acquired: | 32768 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.22 Hz |
| Zero Filling: | 65,536 points |
| Apodization: | Exponential |
| Baseline Correction Method: | Spline |
| Chemical Shift Ref Std: | 0 ppm for DSS |
