Summary of Study ST002409

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001549. The data can be accessed directly via it's Project DOI: 10.21228/M8G99R This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002409
Study TitleSpatial, temporal, and inter-subject variation of the metabolome along the human upper intestinal tract (MS RP positive data)
Study SummaryMost utilization of human diets occurs in the small intestine, which remains largely unstudied. Here, we used a novel non-invasive, ingestible sampling device to probe the spatiotemporal variation of upper intestinal luminal contents during routine daily digestion in 15 healthy subjects. We analyzed 274 intestinal samples and 60 corresponding stool homogenates by combining five metabolomics assays and 16S rRNA sequencing. We identified 1,909 metabolites, including sulfonolipids and novel bile acids. Stool and intestinal metabolomes differed dramatically. Food metabolites displayed known differences and trends in dietary biomarkers, unexpected increases in dicarboxylic acids along the intestinal tract, and a positive association between luminal keto acids and fruit intake. Diet-derived and microbially linked metabolites accounted for the largest inter-subject differences. Interestingly, subjects exhibited large variation in levels of bioactive fatty acid esters of hydroxy fatty acids (FAHFAs) and sulfonolipids. Two subjects who had taken antibiotics within 6 months prior to sampling showed markedly different patterns in these and other microbially related metabolites; from this variation, we identified Blautia species as most likely to be involved in FAHFA metabolism. Thus, in vivo sampling of the human small intestine under physiologic conditions can reveal links between diet, host and microbial metabolism.
University of California, Davis
Last NameFolz
First NameJake
Address1 Shields Ave
Submit Date2022-12-16
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-01-04
Release Version1
Jake Folz Jake Folz application/zip

Select appropriate tab below to view additional metadata details:


Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA2412501978Capsule Type 1
SA2412511546Capsule Type 1
SA2412521475Capsule Type 1
SA2412531550Capsule Type 1
SA2412541479Capsule Type 1
SA2412551936Capsule Type 1
SA2412561486Capsule Type 1
SA2412571973Capsule Type 1
SA2412581542Capsule Type 1
SA2412591470Capsule Type 1
SA2412601932Capsule Type 1
SA2412611558Capsule Type 1
SA2412621458Capsule Type 1
SA2412631457Capsule Type 1
SA2412641559Capsule Type 1
SA2412651988Capsule Type 1
SA2412661462Capsule Type 1
SA2412671538Capsule Type 1
SA2412681466Capsule Type 1
SA2412691985Capsule Type 1
SA2412701982Capsule Type 1
SA2412711970Capsule Type 1
SA2412721507Capsule Type 1
SA2412731506Capsule Type 1
SA2412741526Capsule Type 1
SA2412751962Capsule Type 1
SA2412761508Capsule Type 1
SA2412771957Capsule Type 1
SA2412781953Capsule Type 1
SA2412791949Capsule Type 1
SA2412801522Capsule Type 1
SA2412811502Capsule Type 1
SA2412821945Capsule Type 1
SA2412831494Capsule Type 1
SA2412841491Capsule Type 1
SA2412851940Capsule Type 1
SA2412861533Capsule Type 1
SA2412871966Capsule Type 1
SA2412881944Capsule Type 1
SA2412891530Capsule Type 1
SA2412901498Capsule Type 1
SA2412911561Capsule Type 1
SA2412921929Capsule Type 1
SA2412931920Capsule Type 1
SA2412941909Capsule Type 1
SA2412952011Capsule Type 1
SA2412961425Capsule Type 1
SA2412971906Capsule Type 1
SA2412982008Capsule Type 1
SA2412991437Capsule Type 1
SA2413001436Capsule Type 1
SA2413011435Capsule Type 1
SA2413021917Capsule Type 1
SA2413032014Capsule Type 1
SA2413041417Capsule Type 1
SA2413052017Capsule Type 1
SA2413061414Capsule Type 1
SA2413071413Capsule Type 1
SA2413081418Capsule Type 1
SA2413091914Capsule Type 1
SA2413101422Capsule Type 1
SA2413112015Capsule Type 1
SA2413122016Capsule Type 1
SA2413132005Capsule Type 1
SA2413141434Capsule Type 1
SA2413151898Capsule Type 1
SA2413161897Capsule Type 1
SA2413172001Capsule Type 1
SA2413181442Capsule Type 1
SA2413191993Capsule Type 1
SA2413201902Capsule Type 1
SA2413211924Capsule Type 1
SA2413221997Capsule Type 1
SA2413231450Capsule Type 1
SA2413241446Capsule Type 1
SA2413251518Capsule Type 2
SA2413261555Capsule Type 2
SA2413271899Capsule Type 2
SA2413281527Capsule Type 2
SA2413291946Capsule Type 2
SA2413301520Capsule Type 2
SA2413311519Capsule Type 2
SA2413321915Capsule Type 2
SA2413331562Capsule Type 2
SA2413341523Capsule Type 2
SA2413351557Capsule Type 2
SA2413361925Capsule Type 2
SA2413371539Capsule Type 2
SA2413381933Capsule Type 2
SA2413391928Capsule Type 2
SA2413401937Capsule Type 2
SA2413411543Capsule Type 2
SA2413421921Capsule Type 2
SA2413431547Capsule Type 2
SA2413441537Capsule Type 2
SA2413451551Capsule Type 2
SA2413461531Capsule Type 2
SA2413471903Capsule Type 2
SA2413481918Capsule Type 2
SA2413491910Capsule Type 2
Showing page 1 of 4     Results:    1  2  3  4  Next     Showing results 1 to 100 of 331